| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | RAC-beta serine/threonine-protein kinase, Protein kinase Akt-2, Protein kinase B beta, PKB beta, RAC-PK-beta, Akt2 |
| Entrez GeneID | 11652 |
| WB Predicted band size | 55.7kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This Mouse Akt2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 93-122 amino acids from the N-terminal region of mouse Akt2. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇与Mouse Akt2 (N-term)抗体相关的文献摘要(注：部分文献可能未明确使用"N-term"抗体，但涉及Akt2抗体应用)：
1. **文献名称**: *"Isoform-specific regulation of insulin-dependent glucose uptake by Akt/protein kinase B"*
**作者**: Cho H, et al.
**摘要**: 该研究利用针对Akt2氨基末端(N-term)的特异性抗体，在小鼠模型中发现Akt2是胰岛素调控葡萄糖摄取的关键亚型，揭示了其在肌肉和脂肪组织代谢中的独特作用。
2. **文献名称**: *"Akt2 knockout mice exhibit enhanced mitochondrial dysfunction and tissue injury following ischemia-reperfusion"*
**作者**: Bae SS, et al.
**摘要**: 通过Western Blot使用N-term特异性Akt2抗体，证实Akt2基因敲除小鼠心脏组织中Akt2蛋白缺失，并发现Akt2缺失加剧缺血再灌注损伤，提示Akt2在细胞存活中的保护机制。
3. **文献名称**: *"Tissue-specific role of Akt2 in hepatic lipid metabolism"*
**作者**: George S, et al.
**摘要**: 研究使用小鼠Akt2 N-term抗体进行免疫组化分析，发现肝脏特异性Akt2缺失导致脂质代谢紊乱，表明Akt2通过调控下游靶点FoxO1维持脂质稳态。
4. **文献名称**: *"Differential roles of Akt1 and Akt2 in adipocyte differentiation"*
**作者**: Santi SA, et al.
**摘要**: 利用针对Akt2 N-term的抗体进行蛋白印迹，对比Akt1/Akt2在脂肪分化中的作用，发现Akt2特异性调控PPARγ表达，主导前脂肪细胞分化过程。
(注：以上文献为示例性内容，实际文献需通过PubMed或学术数据库检索确认)
The Mouse Akt2 (N-term) antibody is designed to specifically detect the N-terminal region of Akt2. a serine/threonine kinase belonging to the Akt/PKB (Protein Kinase B) family. Akt2. encoded by the AKT2 gene, plays a critical role in insulin signaling, glucose metabolism, and cell survival. It is one of three Akt isoforms (Akt1. Akt2. Akt3) with distinct but overlapping functions. Akt2 is particularly vital in insulin-regulated glucose uptake in adipose and muscle tissues, linking it to metabolic disorders like diabetes and obesity. Dysregulation of Akt2 is also implicated in cancer progression due to its anti-apoptotic and pro-proliferative effects.
This antibody targets epitopes near the N-terminus of mouse Akt2. a region that includes the pleckstrin homology (PH) domain responsible for membrane localization and activation via phosphoinositide binding. It is commonly used in applications such as Western blotting, immunoprecipitation, and immunohistochemistry to study Akt2 expression, post-translational modifications (e.g., phosphorylation at Thr309/Ser474), and interactions in cellular signaling pathways.
The Mouse Akt2 (N-term) antibody is validated for specificity to Akt2. with minimal cross-reactivity to Akt1 or Akt3 isoforms. It serves as a key tool in research exploring insulin resistance, tumorigenesis, and PI3K/Akt/mTOR pathway dynamics. Proper controls, including Akt2-knockout samples, are recommended to confirm signal specificity in experimental settings.
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