| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Differentially expressed in FDCP 8 homolog, DEF-8, DEF8 |
| Entrez GeneID | 54849 |
| WB Predicted band size | 58.7kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This DEF8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 484-512 amino acids from the C-terminal region of human DEF8. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于DEF8抗体的参考文献示例(注：以下为虚构内容，实际文献需通过学术数据库查询)：
1. **文献名称**: "DEF8 regulates autophagosome formation through its interaction with LC3"
**作者**: Smith A, et al.
**摘要**: 本研究利用DEF8特异性抗体进行免疫共沉淀和免疫荧光实验，证明DEF8通过其C端结构域与LC3蛋白互作，调控自噬体形成，并揭示其在神经元退行性疾病中的潜在作用。
2. **文献名称**: "Development and validation of a high-affinity monoclonal antibody against human DEF8"
**作者**: Chen L, et al.
**摘要**: 报道了一种新型小鼠单克隆抗体的开发，该抗体通过ELISA和Western blot验证对DEF8蛋白的高特异性和敏感性，成功应用于肿瘤组织芯片的免疫组化分析。
3. **文献名称**: "DEF8 expression correlates with poor prognosis in colorectal cancer"
**作者**: Tanaka K, et al.
**摘要**: 通过DEF8抗体对200例结直肠癌样本进行免疫组化分析，发现DEF8高表达与TNM分期正相关，且可作为独立预后标志物，提示其参与Wnt信号通路调控。
4. **文献名称**: "Structural insights into DEF8-antibody recognition by crystallography"
**作者**: Müller R, et al.
**摘要**: 利用X射线晶体学解析了DEF8抗原表位与单克隆抗体的结合模式，揭示了抗体识别DEF8蛋白的关键氨基酸残基，为优化诊断试剂提供了结构基础。
建议通过PubMed、Google Scholar等平台，以"DEF8 antibody"、"DEF8 immune localization"等关键词检索最新文献，或查阅抗体公司(如CST、Abcam)提供的技术文献。
DEF8 (Differentially Expressed in FDCP 8), also known as C16orf88. is a protein implicated in membrane trafficking and endosomal sorting processes. It interacts with components of the ESCRT (Endosomal Sorting Complex Required for Transport) machinery, particularly ESCRT-III, which plays a critical role in multivesicular body formation, cytokinesis, and viral budding. DEF8 contains a MIT (microtubule interacting and trafficking) domain, enabling its association with ESCRT-III subunits like CHMP4B, suggesting its involvement in membrane scission or cargo sorting.
Antibodies targeting DEF8 are primarily used in research to investigate its localization, expression dynamics, and functional roles in cellular processes. Studies have linked DEF8 to immune regulation, autophagy, and cancer progression, though its precise mechanisms remain under exploration. For example, DEF8 may modulate TLR9 signaling in dendritic cells by regulating endosomal trafficking, impacting innate immune responses. In cancer contexts, DEF8 overexpression has been observed in certain tumors, correlating with poor prognosis.
DEF8 antibodies (polyclonal or monoclonal) are validated in applications such as Western blotting, immunofluorescence, and co-immunoprecipitation. Their development aids in elucidating DEF8's contribution to ESCRT-dependent pathways, viral pathogenesis, and diseases involving membrane remodeling defects. However, limited commercial availability and functional data necessitate careful validation for specificity in experimental models.
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