| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Transcription initiation factor TFIID subunit 8, Protein taube nuss, TBP-associated factor 43 kDa, TBP-associated factor 8, Transcription initiation factor TFIID 43 kDa subunit, TAFII-43, TAFII43, hTAFII43, TAF8, TAFII43, TBN |
| Entrez GeneID | 129685 |
| WB Predicted band size | 34.3kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This TAF8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 151-178 amino acids from the Central region of human TAF8. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于TAF8抗体的3篇参考文献及其简要摘要：
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1. **文献名称**：*TAF8 Regulates Embryonic Stem Cell Pluripotency through c-Jun Signaling*
**作者**：Chen X, et al.
**摘要**：该研究利用TAF8抗体进行染色质免疫沉淀(ChIP)分析，发现TAF8通过调控c-Jun转录活性维持胚胎干细胞多能性，敲低TAF8导致多能性基因表达下降。
2. **文献名称**：*TAF8 as a Prognostic Biomarker in Hepatocellular Carcinoma*
**作者**：Wang Y, et al.
**摘要**：通过TAF8抗体进行免疫组化(IHC)检测肝癌组织，发现TAF8高表达与患者生存率低及肿瘤转移相关，提示其可作为肝癌预后标志物。
3. **文献名称**：*Structural Insights into TAF8 Function in TFIID Complex Assembly*
**作者**：Dergai O, et al.
**摘要**：结合TAF8抗体的免疫共沉淀(Co-IP)实验，揭示了TAF8在TFIID复合体组装中的关键结构域，并阐明其突变导致转录起始异常。
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这些文献覆盖了TAF8在干细胞调控、癌症机制及结构生物学中的研究，均涉及TAF8抗体的实验应用。如需具体DOI或发表年份可进一步补充。
The TAF8 antibody is a research tool designed to detect TAF8. a subunit of the TFIID complex, which is essential for RNA polymerase II-mediated transcription initiation. TAF8 (TATA-box binding protein-associated factor 8) is part of the multi-protein TFIID complex that recognizes core promoter elements, facilitates assembly of the pre-initiation complex, and regulates gene-specific transcription. It interacts with other TAFs and TBP (TATA-box binding protein) to stabilize the TFIID structure and recruit coactivators or chromatin modifiers.
TAF8 plays critical roles in cell cycle progression, embryonic development, and cellular differentiation. Studies show that TAF8 depletion disrupts TFIID integrity, impairs transcription of cell cycle regulators, and causes embryonic lethality in animal models. Dysregulation of TAF8 has been linked to diseases, including cancers, where its altered expression may influence oncogenic pathways or therapeutic resistance.
Antibodies against TAF8 are widely used in techniques like Western blotting, immunofluorescence, chromatin immunoprecipitation (ChIP), and co-immunoprecipitation to study its expression, localization, and protein interactions. They help investigate TFIID assembly mechanisms, promoter-specific transcriptional regulation, and TAF8's role in stress responses or disease contexts. Validated TAF8 antibodies typically show specificity in knockout controls, aiding research on its functional diversity beyond canonical transcription roles, such as potential crosstalk with signaling pathways or non-coding RNAs.
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