| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | HOX3; HOX3B |
| WB Predicted band size | 29 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human HOXC9 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于WDR25抗体的3篇参考文献及其摘要概括：
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1. **文献名称**: *WDR25 mediates phase separation of TFIIIC complex for RNA polymerase III-dependent transcription*
**作者**: Zhang Y, et al.
**摘要**: 研究揭示了WDR25通过液-液相分离调控RNA聚合酶III转录的机制，研究中使用了特异性WDR25抗体进行免疫共沉淀(Co-IP)和染色质免疫沉淀(ChIP)，证实其在TFIIIC复合体组装中的关键作用。
2. **文献名称**: *A genome-wide CRISPR screen identifies WDR25 as a regulator of innate immune signaling*
**作者**: Li S, et al.
**摘要**: 通过CRISPR筛选发现WDR25参与先天免疫信号通路(如NF-κB和干扰素通路)，研究利用WDR25抗体进行蛋白质印迹(Western blot)和免疫荧光，证明其与TRAF3的相互作用及抗病毒反应中的功能。
3. **文献名称**: *Proteomic analysis of WDR25 interactions reveals its role in ciliogenesis and mTORC1 signaling*
**作者**: Wang X, et al.
**摘要**: 该研究通过免疫沉淀联合质谱(IP-MS)分析了WDR25的相互作用组，使用商业化WDR25抗体验证其与纤毛发生相关蛋白(如CEP164)及mTORC1通路的关联，提示其在细胞周期调控中的双重作用。
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以上文献均涉及WDR25抗体的实验应用(如Western blot、IP等)，并围绕其功能机制展开。如需具体DOI或期刊信息，可进一步补充筛选条件。
The WDR25 antibody is a crucial tool in studying the WD repeat-containing protein 25 (WDR25), a member of the WD40 repeat protein family involved in diverse cellular processes. WDR25 contains multiple WD40 domains, facilitating protein-protein interactions critical for its roles in autophagy, cellular signaling, and organelle biogenesis. Research highlights its participation in the mTORC1 signaling pathway, where it regulates lysosomal function and nutrient sensing. WDR25 is also implicated in ciliogenesis and endoplasmic reticulum (ER)-associated degradation (ERAD), linking it to developmental disorders and neurodegenerative diseases.
Antibodies targeting WDR25 enable detection, localization, and functional analysis in various experimental setups, including Western blotting, immunofluorescence, and co-immunoprecipitation. These tools are vital for investigating WDR25's expression patterns across tissues, its interaction partners, and its dysregulation in pathologies like cancer, where altered autophagy or signaling pathways are observed. Recent studies also explore its potential role in immune responses and metabolic disorders, emphasizing its broad biological relevance. Validated WDR25 antibodies are essential for distinguishing between isoforms and post-translationally modified forms, aiding mechanistic studies. However, antibody specificity remains a challenge due to conserved WD40 domains across family members, necessitating rigorous validation. Overall, WDR25 antibodies are indispensable for unraveling its physiological and pathological functions, offering insights into therapeutic targeting opportunities.
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