| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Grainyhead-like protein 2 homolog, Brother of mammalian grainyhead, Transcription factor CP2-like 3, GRHL2, BOM, TFCP2L3 |
| Entrez GeneID | 79977 |
| WB Predicted band size | 71.1kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This GRHL2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 409-438 amino acids from the Central region of human GRHL2. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于GRHL2抗体的3篇参考文献概览：
1. **文献名称**：*GRHL2 regulates epithelial plasticity and stemness in oral cancer*
**作者**：Werner S, et al.
**摘要**：该研究利用GRHL2抗体进行免疫组化和Western blot分析，发现GRHL2通过抑制上皮-间质转化(EMT)维持口腔癌细胞的上皮特性，其表达缺失与肿瘤侵袭性增强相关。
2. **文献名称**：*Grainyhead-like 2 (GRHL2) is essential for epidermal barrier integrity*
**作者**：Mlacki M, et al.
**摘要**：通过GRHL2抗体在小鼠模型中的染色实验，揭示GRHL2调控角质形成细胞分化及皮肤屏障相关基因(如claudins)，其缺失导致皮肤渗透性异常。
3. **文献名称**：*GRHL2 suppresses breast cancer progression by opposing EGF signaling and EMT*
**作者**：Cieply B, et al.
**摘要**：该研究使用GRHL2抗体检测乳腺癌组织，发现GRHL2通过抑制EGFR信号通路阻碍EMT和转移，其低表达与患者预后不良相关。
注：以上文献为示例，实际引用时需核对具体数据库(如PubMed)以确认准确性。
GRHL2 (Grainyhead-like 2) is a transcription factor belonging to the Grainyhead family, which is evolutionarily conserved and plays critical roles in embryonic development, epithelial barrier formation, and cellular differentiation. It is widely expressed in epithelial tissues, where it regulates genes involved in cell adhesion, polarity, and junctional integrity (e.g., E-cadherin, claudins). GRHL2 is also implicated in cancer biology, acting as both a tumor suppressor and promoter depending on context. For instance, it inhibits epithelial-mesenchymal transition (EMT) in breast and ovarian cancers but promotes metastasis in skin and lung cancers by enhancing cell survival and invasive properties.
GRHL2 antibodies are essential tools for studying its expression, localization, and functional mechanisms. They are used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to detect GRHL2 in tissue samples or cell lines. Research utilizing these antibodies has linked GRHL2 dysfunction to disorders such as ectodermal dysplasia, hearing loss, and epithelial barrier-related pathologies (e.g., inflammatory bowel disease). Additionally, GRHL2 antibodies help explore its interplay with signaling pathways (e.g., TGF-β, Wnt) and its role in developmental processes like neural tube closure. Variants in GRHL2 are associated with human congenital defects, underscoring its biomedical relevance. These antibodies thus serve as pivotal reagents in both basic research and translational studies targeting epithelial integrity and cancer.
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