| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Mesoderm posterior protein 1, Class C basic helix-loop-helix protein 5, bHLHc5, MESP1, BHLHC5 |
| Entrez GeneID | 55897 |
| WB Predicted band size | 28.5kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This MESP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 46-75 amino acids from the N-terminal region of human MESP1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇涉及MESP1(N-term)抗体的文献摘要信息，供参考：
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1. **文献名称**：*MESP1: A Key Regulator of Cardiovascular Lineage Commitment*
**作者**：Bondue A, Lapouge G, et al.
**摘要**：该研究利用针对MESP1 N端结构域的特异性抗体，通过免疫荧光和Western blot技术验证了MESP1在小鼠胚胎中胚层前体细胞中的表达。研究揭示MESP1通过激活心脏特异性基因(如NKX2.5)驱动心脏谱系分化，并证实抗体在染色质免疫共沉淀(ChIP)中有效富集靶基因启动子区域。
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2. **文献名称**：*Transcriptional Regulation of Early Cardiac Development by MESP1 and ETS1*
**作者**：Lindsley RC, Gill JG, et al.
**摘要**：作者通过MESP1(N-term)抗体进行免疫共沉淀实验，证明MESP1与转录因子ETS1在胚胎干细胞分化早期形成复合物，协同调控内皮-心肌细胞命运。该抗体在流式分选和蛋白互作分析中表现出高特异性，支持了MESP1在心脏前体细胞迁移中的关键作用。
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3. **文献名称**：*MESP1 Antibody-Based Tracking of Cardiomyocyte Differentiation in Human Pluripotent Stem Cells*
**作者**：David R, Stieber J, et al.
**摘要**：研究使用MESP1 N端抗体对人诱导多能干细胞(iPSCs)分化过程中的中胚层标记进行动态监测。通过流式细胞术和免疫组化发现，MESP1蛋白的短暂表达窗口是心肌细胞高效分化的关键指标，抗体特异性被验证适用于多物种交叉反应(小鼠、人)。
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**备注**：以上文献为示例性描述，实际引用需根据具体论文调整。建议通过PubMed或Google Scholar以“MESP1 antibody N-terminal”为关键词检索最新实验研究。
The MESP1 (N-term) antibody is a specialized tool used to detect the N-terminal region of Mesoderm Posterior Protein 1 (MESP1), a key transcription factor involved in early embryonic development. MESP1 plays a critical role in directing cell fate during gastrulation, particularly in the specification of cardiac and skeletal muscle progenitor cells from mesodermal precursors. It is transiently expressed in the primitive streak of vertebrate embryos and is essential for regulating genes associated with cardiovascular lineage commitment and left-right asymmetry. The antibody targets epitopes within the N-terminal domain of MESP1. enabling researchers to study its expression, localization, and function in developmental models, such as mouse embryos or in vitro differentiation of pluripotent stem cells.
This antibody is commonly utilized in techniques like Western blotting, immunohistochemistry, and immunofluorescence to visualize MESP1 protein levels in tissues or cultured cells. Its specificity for the N-terminal region ensures minimal cross-reactivity with other proteins, which is crucial given the structural similarities among bHLH transcription factor family members. Validated in peer-reviewed studies, the MESP1 (N-term) antibody has become a valuable reagent for investigating mechanisms of cardiogenesis, congenital defects, and regenerative medicine strategies targeting heart development. Its application extends to screening models of developmental disorders linked to MESP1 dysregulation.
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