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Rabbit Polyclonal POFUT2 Antibody

  • 中文名： POFUT2抗体
  • 别    名： GDP-fucose protein O-fucosyltransferase 2, Peptide-O-fucosyltransferase 2, O-FucT-2, POFUT2, C21orf80, FUT13, KIAA0958
货号: IPDX31179
Price： ￥1180
数量：
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验证与应用

应用及物种
WB 1/1000 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 1/100-1/500 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesGDP-fucose protein O-fucosyltransferase 2, Peptide-O-fucosyltransferase 2, O-FucT-2, POFUT2, C21orf80, FUT13, KIAA0958
Entrez GeneID23275
WB Predicted band size50.0kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman
ImmunogenThis POFUT2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 228-257 amino acids from the Central region of human POFUT2.
FormulationPurified antibody in PBS with 0.05% sodium azide.

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参考文献

以下是关于POFUT2抗体的3篇参考文献的简要信息(注：以下文献为示例，实际引用需根据真实文献调整)：

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1. **文献名称**：*POFUT2-mediated O-glycosylation is required for ADAMTS13 secretion and vascular development*

**作者**：Wang Y. et al. (2018)

**摘要**：本研究揭示了POFUT2在ADAMTS13蛋白分泌中的作用，通过CRISPR/Cas9敲除细胞模型，利用POFUT2特异性抗体进行Western blot分析，发现其缺失导致ADAMTS13糖基化异常，影响血管生成。

2. **文献名称**：*Antibody-based detection of POFUT2 in colorectal cancer tissues*

**作者**：Smith J.L. et al. (2020)

**摘要**：开发了一种高灵敏度的兔源多克隆POFUT2抗体，用于免疫组织化学检测结直肠癌组织中POFUT2的表达水平升高，提示其可能作为肿瘤进展的生物标志物。

3. **文献名称**：*Functional analysis of POFUT2 in Notch signaling using a novel monoclonal antibody*

**作者**：Zhang H. et al. (2019)

**摘要**：报道了一种新型小鼠单克隆POFUT2抗体的开发，通过免疫荧光和流式细胞术验证其在多种细胞系中的特异性，证实POFUT2缺失导致Notch配体功能异常，影响细胞分化。

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如需实际文献，建议通过PubMed(https://pubmed.ncbi.nlm.nih.gov/)或Google Scholar检索关键词“POFUT2 antibody”获取最新研究。

背景信息

The POFUT2 (Protein O-fucosyltransferase 2) antibody is a tool used to study the enzyme responsible for catalyzing O-fucosylation, a post-translational modification of proteins containing thrombospondin type-1 (TSP1) repeats. POFUT2 specifically transfers fucose residues to serine or threonine residues within these repeats, a process critical for the secretion and functional maturation of TSP1-containing proteins, such as ADAMTS family proteases and certain extracellular matrix components. Dysregulation of POFUT2-mediated O-fucosylation has been linked to developmental disorders, connective tissue diseases, and cancer progression. For instance, mutations in POFUT2 are associated with autosomal recessive Dowling-Degos disease, characterized by skin hyperpigmentation and epidermal defects. The POFUT2 antibody enables researchers to detect and quantify POFUT2 expression in tissues or cell lines, assess its subcellular localization (primarily endoplasmic reticulum), and investigate its role in disease models. It is widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence. Recent studies also explore POFUT2's involvement in cancer metastasis and stem cell maintenance, highlighting its potential as a therapeutic target. Commercial POFUT2 antibodies are typically raised in rabbits or mice against specific peptide epitopes, with validation in knockout controls to ensure specificity.

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