| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Carbohydrate sulfotransferase 8, 282-, GalNAc-4-O-sulfotransferase 1, GalNAc-4-ST1, GalNAc4ST-1, N-acetylgalactosamine-4-O-sulfotransferase 1, CHST8 |
| Entrez GeneID | 64377 |
| WB Predicted band size | 48.8kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This CHST8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 219-247 amino acids from the Central region of human CHST8. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于CHST8抗体的假设性参考文献示例(内容基于研究领域常见方向模拟，非真实文献)：
1. **《CHST8在结直肠癌中的表达及其临床意义》**
- 作者：Zhang L, Wang Y, et al.
- 摘要：本研究通过免疫组化(使用CHST8抗体)和Western blot分析，发现CHST8在结直肠癌组织中高表达，且与患者预后不良相关。实验表明CHST8可能通过调控硫酸软骨素合成促进肿瘤侵袭。
2. **《CHST8缺失对小鼠大脑硫酸软骨素分布的影响》**
- 作者：Tanaka K, Suzuki H.
- 摘要：利用CHST8特异性抗体进行免疫荧光染色，发现CHST8基因敲除小鼠大脑皮层硫酸软骨素水平显著降低，提示CHST8在中枢神经系统细胞外基质修饰中起关键作用。
3. **《CHST8与骨关节炎软骨退变的关联研究》**
- 作者：Chen X, Liu R, et al.
- 摘要：通过Western blot(基于CHST8抗体)和qRT-PCR技术，证实骨关节炎患者软骨组织中CHST8表达下调，导致硫酸软骨素合成异常，可能加速软骨退化进程。
4. **《CHST8抗体在胰腺癌细胞系功能筛选中的应用》**
- 作者：Müller S, et al.
- 摘要：研究利用CHST8抗体进行流式细胞术和免疫沉淀，发现抑制CHST8可降低胰腺癌细胞迁移能力，提示其作为潜在治疗靶点的价值。
(注：以上文献为示例性质，实际文献需通过学术数据库检索获取。)
The CHST8 antibody targets the carbohydrate sulfotransferase 8 (CHST8) protein, a member of the sulfotransferase family involved in post-translational modification of glycoproteins and glycolipids. CHST8 catalyzes the transfer of sulfate groups to specific carbohydrate substrates, particularly glycosaminoglycans (GAGs), influencing cellular processes like cell adhesion, signaling, and extracellular matrix organization. It is primarily expressed in the Golgi apparatus and plays a role in sulfation of chondroitin sulfate, a critical component for neural development, immune regulation, and tumor metastasis.
CHST8 antibodies are essential tools for studying the protein's expression, localization, and function in biological systems. They are commonly used in techniques such as Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to investigate CHST8's role in diseases like cancer, neurological disorders, and inflammatory conditions. For instance, altered CHST8 expression has been linked to tumor progression due to its impact on extracellular matrix remodeling and cell migration.
These antibodies are typically produced in hosts like rabbits or mice using immunogenic peptides or recombinant CHST8 proteins. Validation includes specificity tests via knockout cell lines or siRNA knockdown. Researchers rely on CHST8 antibodies to explore therapeutic targets or biomarkers, particularly in contexts where sulfation pathways are dysregulated. Their applications extend to developmental biology, emphasizing CHST8's contribution to tissue morphogenesis and homeostasis.
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