| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Histone H2B type 1-C/E/F/G/I, Histone H2B1 A, Histone H2Ba, H2B/a, Histone H2Bg, H2B/g, Histone H2Bh, H2B/h, Histone H2Bk, H2B/k, Histone H2Bl, H2B/l, HIST1H2BC, H2BFL |
| Entrez GeneID | 3017;8339;8343;8344;8346;8347 |
| WB Predicted band size | 13.9kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This HIST1H2BC/HIST1H2BF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human HIST1H2BC/HIST1H2BF. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于HIST1H2BC/HIST1H2BF (N-term)抗体的3篇参考文献：
1. **"Histone H2B isoforms in transcriptional regulation"**
- **作者**: Smith A, et al.
- **摘要**: 研究通过N端特异性抗体分析HIST1H2BC和HIST1H2BF在染色质动态中的作用，发现二者通过组蛋白修饰影响基因转录调控，抗体用于ChIP-seq验证启动子结合模式。
2. **"Epigenetic regulation by histone H2B variants in cancer"**
- **作者**: Lee J, et al.
- **摘要**: 利用HIST1H2BF (N-term)抗体进行免疫印迹和免疫荧光实验，证明该异构体在癌细胞中异常表达，与DNA修复异常和表观遗传失调相关。
3. **"Characterization of histone H2B N-terminal antibodies for chromatin studies"**
- **作者**: Brown K, et al.
- **摘要**: 验证多种H2B家族N端抗体的特异性，包括HIST1H2BC抗体，证明其在核小体定位和组蛋白置换分析中的应用价值，强调抗体对功能研究的重要性。
(注：以上文献为示例性内容，实际引用需根据具体研究补充真实文献信息。)
The HIST1H2BC/HIST1H2BF (N-term) antibody targets the N-terminal region of histone H2B type 1 proteins encoded by the HIST1H2BC and HIST1H2BF genes, which are part of the histone H2B family. These histones are core components of nucleosomes, playing essential roles in chromatin structure and epigenetic regulation. The HIST1 cluster, located on chromosome 6p22.2 in humans, contains multiple histone genes expressed during the S phase of the cell cycle to support DNA replication and packaging.
The N-terminal regions of H2B histones are subject to post-translational modifications (PTMs), such as phosphorylation, acetylation, and ubiquitination, which influence chromatin dynamics, transcriptional regulation, and DNA repair. Antibodies specific to this region are valuable tools for studying these modifications and their roles in cellular processes. The HIST1H2BC/HIST1H2BF (N-term) antibody is commonly used in techniques like ChIP-seq, Western blotting, and immunofluorescence to investigate histone modification patterns, nucleosome organization, and epigenetic mechanisms in diseases like cancer or developmental disorders.
This antibody’s specificity for the N-terminus ensures recognition of conserved epitopes across H2B variants, though cross-reactivity with other HIST1H2B isoforms may occur due to sequence homology. Validation typically includes testing on recombinant proteins or knockout models to confirm target selectivity. Its applications span basic research in chromatin biology, as well as clinical studies exploring histone modification biomarkers.
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