| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | RNA-binding protein 28, RNA-binding motif protein 28, RBM28 |
| Entrez GeneID | 55131 |
| WB Predicted band size | 85.7kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This RBM28 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 62-91 amino acids from the N-terminal region of human RBM28. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于RBM28 (N-term)抗体的参考文献示例(注：以下内容为模拟示例，实际文献需通过学术数据库检索获取)：
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1. **文献名称**: *RBM28 regulates ribosomal RNA processing through its N-terminal domain*
**作者**: Zhang L, et al.
**摘要**: 本研究利用针对RBM28 N端结构域的特异性抗体，通过免疫沉淀和Western blot分析，揭示了RBM28在核仁中参与pre-rRNA加工的作用机制。结果显示N端抗体能有效识别内源性RBM28蛋白，并证实其与核糖体生物合成复合物的相互作用。
2. **文献名称**: *Characterization of a novel polyclonal antibody targeting the N-terminus of RBM28*
**作者**: Johnson M, et al.
**摘要**: 文章报道了一种新型兔源多克隆抗体的开发与验证，该抗体靶向RBM28的N端(氨基酸1-150)。实验表明该抗体适用于免疫荧光和免疫组化，成功用于检测人类细胞系和小鼠组织切片中RBM28的亚细胞定位。
3. **文献名称**: *RBM28 mutations disrupt RNA splicing and cause congenital anomalies*
**作者**: Smith KA, et al.
**摘要**: 本研究通过CRISPR/Cas9构建RBM28突变细胞模型，结合N端特异性抗体进行蛋白质表达分析，发现RBM28的N端结构域对其在mRNA剪接中的功能至关重要，突变导致剪接因子结合异常及发育缺陷。
4. **文献名称**: *Proteomic analysis of RBM28-interacting partners using N-terminal specific antibodies*
**作者**: Chen H, et al.
**摘要**: 利用抗RBM28 N端抗体进行免疫共沉淀-质谱联用技术，鉴定了RBM28在Hela细胞中的相互作用蛋白网络，强调了其在RNA代谢和应激反应中的多功能性。
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**建议**：实际文献可通过PubMed、Google Scholar等平台以关键词“RBM28 antibody N-terminal”或“RBM28 epitope mapping”检索，并筛选涉及抗体开发、功能研究或应用技术的论文。
The RBM28 (N-term) antibody is designed to target the N-terminal region of RNA-binding motif protein 28 (RBM28), a nucleolar matrix protein involved in ribosomal RNA (rRNA) processing and ribosome biogenesis. RBM28 contains RNA recognition motifs (RRMs) and plays a critical role in cell cycle progression, stress response, and maintaining nucleolar integrity. It is ubiquitously expressed, with high levels in proliferating cells, and interacts with other nucleolar proteins to ensure proper maturation of 18S and 28S rRNA subunits.
This antibody is commonly used in research to study RBM28's expression, localization, and function via techniques like Western blotting, immunofluorescence, and immunohistochemistry. By targeting the N-terminal domain, it helps detect full-length RBM28 and distinguish it from potential truncated isoforms or cleavage products. Dysregulation of RBM28 has been linked to human diseases, including alopecia, neurological defects, and endocrinopathy (ANE) syndrome, where mutations disrupt its role in rRNA processing, leading to nucleolar stress and cell death.
Validated in human and rodent models, the RBM28 (N-term) antibody serves as a key tool for investigating nucleolar biology, ribosomopathies, and cellular responses to DNA damage or metabolic stress. Its specificity for the conserved N-terminal region ensures reliable detection across species, aiding mechanistic studies of RBM28-related pathologies.
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