关键信息
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基因名
ksdD
- 应用
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别名
3-keto-Delta(4)-steroid Delta(1)-dehydrogenase;KSDD;3-oxo-Delta(4)-steroid 1-dehydrogenase;KSTD
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种属
Mycolicibacterium smegmatis
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表达系统
E. coli
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标签
N- His & C- Myc
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
A0R4S9
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表达区间
1-569aa
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分子量
68.9 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
KSDD (Kinetoplastida Surface-Defined Domain) recombinant proteins have garnered significant interest in the field of molecular biology and parasitology due to their potential applications in vaccine development and therapeutic interventions against diseases caused by Kinetoplastida, such as African sleeping sickness and Chagas disease. These proteins are derived from the unique mid-outer membrane structures of trypanosomes and other related parasites, which play crucial roles in host-pathogen interactions. The study of KSDD recombinant proteins aims to elucidate their structure, function, and immunogenic properties, thereby providing insights into the immune evasion mechanisms of these parasites. Recent advances in protein engineering and expression systems have enabled researchers to produce KSDD recombinant proteins in sufficient quantities for rigorous experimental analysis. By characterizing these proteins, scientists hope to identify novel targets for diagnostics and vaccines that could effectively boost the immune response against Kinetoplastida species. Moreover, understanding the biochemical pathways and surface presentations of KSDD proteins may aid in the design of inhibitors that disrupt parasite function. Therefore, ongoing research into KSDD recombinant proteins is not only relevant for basic biological understanding but also pivotal for developing innovative strategies to combat Kinetoplastida-related diseases, which pose significant public health challenges, particularly in endemic regions of Africa and South America.
