| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Calcium-binding mitochondrial carrier protein Aralar1, Mitochondrial aspartate glutamate carrier 1, Solute carrier family 25 member 12, SLC25A12, ARALAR1 |
| Entrez GeneID | 8604 |
| WB Predicted band size | 74.8kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This SLC25A12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human SLC25A12. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
+ +
1. **"Molecular cloning and characterization of the human mitochondrial aspartate/glutamate carrier SLC25A12", Palmieri et al. (2006)**
- **摘要**：该研究克隆了人源SLC25A12基因，分析了其编码蛋白AGC1的线粒体天冬氨酸/谷氨酸转运功能，并开发了针对N端的抗体，用于Western blot验证组织特异性表达。
2. **"AGC1 deficiency associated with global cerebral hypomyelination", Wibom et al. (2009)**
- **摘要**：通过SLC25A12(N-Term)抗体进行免疫印迹和免疫组化，发现AGC1缺失导致脑白质发育异常，揭示了其在髓鞘形成中的关键作用。
3. **"Mitochondrial carrier family: Structure and functional roles", Palmieri (2011)**
- **摘要**：综述线粒体载体蛋白家族的结构与功能，提及SLC25A12的N端特异性抗体在解析其亚细胞定位及病理机制中的应用。
4. **"SLC25A12 and ASD-associated 16p11.2 deletion", Lepagnol-Bestel et al. (2009)**
- **摘要**：利用N-Term抗体检测自闭症患者脑组织中SLC25A12表达异常，探讨其与16p11.2缺失综合征的潜在关联。
每篇均涉及SLC25A12抗体的实验应用(如蛋白表达验证、疾病机制研究)，聚焦N端抗原表位。
The SLC25A12 (N-Term) antibody is a specialized tool used to detect the N-terminal region of the solute carrier family 25 member 12 (SLC25A12) protein, also known as aralar1. SLC25A12 encodes a calcium-binding mitochondrial carrier protein localized in the inner mitochondrial membrane. It functions as an aspartate/glutamate exchanger, playing a critical role in the malate-aspartate shuttle, which facilitates the transfer of reducing equivalents into mitochondria for ATP production and supports cytosolic NAD⁺ regeneration. This protein is highly expressed in the brain, skeletal muscle, and heart, and is implicated in neuronal development and calcium signaling.
The antibody targeting the N-terminal region is particularly useful for distinguishing SLC25A12 from its isoform SLC25A13 (aralar2), as sequence divergence occurs in this domain. Researchers employ this antibody in techniques like Western blotting, immunohistochemistry, and immunofluorescence to study protein expression, subcellular localization, and functional alterations in diseases. Dysregulation of SLC25A12 has been linked to autism spectrum disorders (ASD), neurodegenerative conditions, and metabolic syndromes, making the antibody a valuable reagent for both basic research and clinical investigations. Its specificity ensures accurate detection, aiding in elucidating the protein's role in mitochondrial dysfunction and related pathologies.
×
