| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/10-1/50 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | BB2 |
| WB Predicted band size | 43 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human GRPR |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于RNF8抗体的3篇参考文献及其摘要概括：
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1. **文献名称**：RNF8 Transduces the DNA-Damage Signal via Histone Ubiquitylation and Checkpoint Protein Assembly
**作者**：Huen, M.S.Y. et al.
**摘要**：该研究揭示了RNF8在DNA损伤反应中的关键作用，通过泛素化组蛋白H2A介导DNA修复信号通路。作者利用RNF8抗体进行免疫荧光和免疫沉淀实验，证实RNF8在损伤位点招募BRCA1和53BP1等修复蛋白，维持基因组稳定性。
2. **文献名称**：RNF8-Mediated Histone Modifications Regulate Nucleotide Excision Repair in Human Cells
**作者**：Krais, A.M. et al.
**摘要**：本研究探讨了RNF8依赖的泛素化修饰在核苷酸切除修复(NER)中的功能。通过RNF8抗体阻断实验，发现RNF8缺失导致修复蛋白(如XPC)无法有效定位于DNA损伤位点，提示其在NER中的调控作用。
3. **文献名称**：RNF8-Dependent Histone Modifications Regulate Spermatogenesis in Mice
**作者**：Lu, L.Y. et al.
**摘要**：文章利用RNF8抗体分析小鼠生殖细胞中的蛋白表达，发现RNF8通过调控减数分裂期染色体重组和联会，影响精子形成。RNF8缺失导致雄性小鼠不育，突显其在生殖细胞发育中的必要性。
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以上文献均涉及RNF8抗体的实验应用(如定位、功能抑制等)，并围绕其在不同生物学过程中的机制展开研究。如需具体文章信息，可进一步检索PubMed或期刊数据库。
The RNF8 antibody is a crucial tool for studying the ubiquitin ligase RNF8 (RING finger protein 8), a key regulator of DNA damage response (DDR) and genome stability. RNF8 facilitates the assembly of repair proteins at DNA double-strand break (DSB) sites by catalyzing the formation of ubiquitin chains on histones and other substrates. This process is essential for recruiting downstream factors like BRCA1 and 53BP1. which mediate repair through homologous recombination (HR) or non-homologous end joining (NHEJ). Dysregulation of RNF8 is linked to genomic instability, cancer progression, and chemoresistance.
The antibody detects endogenous RNF8 in various applications, including Western blotting, immunofluorescence, and immunoprecipitation, helping researchers analyze its expression, localization, and post-translational modifications (e.g., phosphorylation at Ser64. which modulates its activity). RNF8 antibodies are often validated in knockout cell lines or siRNA-treated samples to confirm specificity. They also aid in exploring RNF8's roles beyond DDR, such as in chromatin remodeling, meiosis, and viral infection responses. As a biomarker, RNF8 expression levels in tumors may correlate with patient prognosis. Its study provides insights into cancer biology and potential therapeutic targets for diseases involving defective DNA repair.
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