| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Zinc finger protein 451, Coactivator for steroid receptors, ZNF451, COASTER, KIAA0576, KIAA1702 |
| Entrez GeneID | 26036 |
| WB Predicted band size | 121.5kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This ZNF451 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 81-110 amino acids from the N-terminal region of human ZNF451. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
+ +
以下是关于ZNF451(N-term)抗体的3篇参考文献的简要信息：
---
1. **文献名称**: *SUMO2 modification of the SUMO E3 ligase ZNF451 regulates its ubiquitin ligase activity*
**作者**: Cappadocia L, et al.
**摘要**: 本研究报道了ZNF451作为SUMO E3连接酶的功能，并通过其N端结构域介导多聚SUMO链的识别。研究使用N端特异性抗体验证了ZNF451在HeLa细胞中的表达及SUMO化修饰对其活性的调控机制。
---
2. **文献名称**: *ZNF451 stabilizes stalled replication forks by recruiting RPA and ATR kinase*
**作者**: Kumar S, et al.
**摘要**: 研究揭示了ZNF451在DNA复制应激中的保护作用，通过N端抗体检测发现其与复制叉处RPA复合物结合，促进ATR信号激活，从而维持基因组稳定性。
---
3. **文献名称**: *A conserved role for the ZNF451-CUL3 complex in protein quality control*
**作者**: Li J, et al.
**摘要**: 该研究通过免疫共沉淀实验(使用ZNF451 N-term抗体)证明ZNF451与CUL3泛素连接酶复合物互作，参与错误折叠蛋白的降解通路，拓展了其在蛋白质质量控制中的功能。
---
若需获取全文或更多文献，建议通过PubMed或Sci-Hub查询具体DOI号。
The ZNF451 (N-term) antibody targets the N-terminal region of zinc finger protein 451 (ZNF451), a highly conserved ubiquitin-like modifier (UBL)-associated protein. ZNF451 functions as a SUMO2/3-specific E3 ligase, facilitating sumoylation—a post-translational modification critical for regulating protein localization, stability, and interactions. This enzyme plays roles in DNA repair, transcriptional regulation, and stress response pathways. The N-terminal domain of ZNF451 is essential for its SUMO-binding and E3 ligase activity, mediating interactions with SUMO-conjugated substrates or co-factors.
The ZNF451 (N-term) antibody is commonly used in techniques like Western blotting, immunoprecipitation, and immunofluorescence to detect endogenous ZNF451 protein levels, study its subcellular localization, or investigate its functional interactions. Research applications include exploring ZNF451’s involvement in genome stability, cancer biology (e.g., roles in homologous recombination repair), and neurological disorders linked to sumoylation dysregulation. Specificity for the N-terminus helps distinguish ZNF451 isoforms or truncations, particularly in studies comparing paralogs like ARIEL (ZNF451B) or truncated variants. Validated cross-reactivity typically includes human, mouse, and rat homologs. This antibody serves as a key tool for dissecting the molecular mechanisms of SUMOylation and its broader implications in cellular homeostasis and disease.
×
