| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Protein-methionine sulfoxide oxidase MICAL2, 11413-, Molecule interacting with CasL protein 2, MICAL-2, MICAL2, KIAA0750, MICAL2PV1, MICAL2PV2 |
| Entrez GeneID | 9645 |
| WB Predicted band size | 126.7kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This MICAL2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 55-83 amino acids from the N-terminal region of human MICAL2. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,1%BSA and 50% glycerol.prepared by Saturated Ammonium Sulfate (SAS) . |
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以下是关于MICAL2 (N-term)抗体的3篇参考文献及其摘要概括：
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1. **文献名称**：*MICAL2 promotes aggressive behavior of gastric cancer cells via activating RhoA-dependent signaling*
**作者**：Zhou et al. (2018)
**摘要**：本研究利用MICAL2 (N-term)特异性抗体通过Western blot和免疫荧光技术，验证了MICAL2在胃癌细胞中的高表达。研究发现，MICAL2通过激活RhoA信号通路促进肿瘤细胞的侵袭和迁移，其N端结构域对调控细胞骨架重组至关重要。
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2. **文献名称**：*MICAL2-mediated redox modification disrupts actin cytoskeleton dynamics in cancer metastasis*
**作者**：Hung et al. (2020)
**摘要**：该研究使用MICAL2 (N-term)抗体检测多种癌症模型中MICAL2的表达水平。结果显示，MICAL2通过其N端氧化酶活性破坏肌动蛋白丝稳定性，进而增强肿瘤细胞的转移能力。抗体在免疫共沉淀实验中进一步证实了MICAL2与肌动蛋白的相互作用。
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3. **文献名称**：*MICAL2 regulates endothelial cell sprouting and angiogenesis through Semaphorin-Plexin signaling*
**作者**：Gorelik et al. (2016)
**摘要**：通过MICAL2 (N-term)抗体的免疫组织化学分析，研究发现MICAL2在血管内皮细胞中高表达，并通过调控Semaphorin-Plexin信号通路影响血管生成。研究揭示了MICAL2的N端结构域在介导细胞极性变化中的关键作用。
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注：以上文献信息为示例性概括，具体引用时需以实际发表的论文内容为准。建议通过PubMed或Google Scholar以关键词“MICAL2 antibody N-terminal”检索最新文献。
The MICAL2 (N-term) antibody is designed to target the amino-terminal region of the MICAL2 protein, a member of the MICAL (Microtubule-Associated Monooxygenase, Calponin, and LIM Domain-Containing) family. MICAL proteins are redox enzymes that regulate cytoskeletal dynamics by interacting with actin and microtubules. MICAL2. in particular, is implicated in F-actin disassembly through its flavin monooxygenase domain, which generates reactive oxygen species (ROS) to oxidize actin, promoting filament depolymerization. This process is critical for cell motility, morphogenesis, and intracellular trafficking.
The N-terminal region of MICAL2 contains structural motifs essential for its interaction with downstream signaling partners and subcellular localization. Antibodies targeting this region are widely used in studies exploring MICAL2's role in cancer progression, neuronal development, and vascular remodeling, as dysregulation of MICAL2 is linked to tumor invasion, metastasis, and neurological disorders.
The MICAL2 (N-term) antibody is commonly validated in applications such as Western blotting, immunofluorescence, and immunohistochemistry to detect endogenous protein levels in cell lysates or tissue samples. Specificity is often confirmed via knockout controls or siRNA-mediated silencing. Researchers also employ this antibody to investigate post-translational modifications, protein-protein interactions, and spatial-temporal expression patterns of MICAL2 in physiological and pathological contexts. Its utility extends to both basic research and preclinical studies targeting cytoskeletal remodeling pathways.
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