| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/10-1/50 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Probable ATP-dependent RNA helicase DDX17, DEAD box protein 17, DEAD box protein p72, RNA-dependent helicase p72, DDX17 |
| Entrez GeneID | 10521 |
| WB Predicted band size | 80.3kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This DDX17 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 128-155 amino acids from the N-terminal region of human DDX17. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇涉及DDX17(N-term)抗体的参考文献及其简要摘要：
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1. **"DEAD-box helicase DDX17 regulates pre-mRNA splicing and genome stability"**
*作者：Yang et al., 2020*
摘要：该研究利用DDX17的N端特异性抗体，通过免疫沉淀和蛋白质组学分析，发现DDX17与剪接体复合物相互作用，并通过调控pre-mRNA剪接维持基因组稳定性。抗体用于验证DDX17在细胞核内的定位及其与其他剪接因子的共定位。
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2. **"DDX17 coordinates liquid-liquid phase separation to promote transcriptional activation"**
*作者：Zhang et al., 2021*
摘要：研究通过N端抗体进行染色质免疫沉淀(ChIP)和免疫荧光实验，证明DDX17通过相分离机制促进转录激活复合物的形成。抗体特异性验证显示其能有效识别内源性DDX17的N端结构域，并揭示其在乳腺癌中的功能失调。
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3. **"A genome-wide CRISPR screen identifies DDX17 as a critical host factor for influenza virus replication"**
*作者：Li et al., 2019*
摘要：作者使用DDX17(N-term)抗体进行Western blot和免疫共沉淀实验，证实DDX17与流感病毒RNA结合，并参与病毒复制周期。抗体的特异性通过siRNA敲低实验验证，支持其在宿主-病毒互作研究中的应用。
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这些文献均使用了针对DDX17 N端区域的抗体进行功能研究，涵盖剪接调控、相分离及病毒复制机制等领域。如需具体抗体品牌或实验细节，可进一步查阅原文方法部分。
The DDX17 (N-term) antibody is a tool used to detect the N-terminal region of DEAD-box helicase 17 (DDX17), a member of the DEAD-box RNA helicase family. DDX17 plays critical roles in RNA metabolism, including pre-mRNA splicing, ribosome biogenesis, miRNA processing, and transcriptional regulation. It binds RNA in an ATP-dependent manner, facilitating RNA unwinding and remodeling. The protein contains conserved DEAD (Asp-Glu-Ala-Asp) motifs essential for its helicase activity and interacts with multiple partners, such as p53 and estrogen receptor alpha, linking RNA processing to broader cellular functions like stress responses and hormone signaling.
Antibodies targeting the N-terminal region of DDX17 are valuable for studying its localization, expression, and molecular interactions. The N-terminal domain is implicated in protein-protein interactions and regulatory functions, making this antibody particularly useful in co-immunoprecipitation, immunofluorescence, and Western blot assays. Researchers often employ it to investigate DDX17's involvement in diseases, including cancer, viral infections (e.g., HIV, influenza), and neurological disorders, where aberrant RNA metabolism is a hallmark. Validation of DDX17 (N-term) antibodies typically includes knockout cell line controls to confirm specificity, given the high homology among DEAD-box family members. Its application has advanced understanding of DDX17's dual roles in maintaining genomic stability and regulating gene expression networks.
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