| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/10-1/50 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Carbonic anhydrase 5B, mitochondrial, Carbonate dehydratase VB, Carbonic anhydrase VB, CA-VB, CA5B |
| Entrez GeneID | 11238 |
| WB Predicted band size | 36.4kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This CA5B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 253-283 amino acids from the C-terminal region of human CA5B. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,1%BSA and 50% glycerol.prepared by Saturated Ammonium Sulfate (SAS) . |
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以下是关于CA5B抗体的3篇假设性参考文献示例，供参考：
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1. **文献名称**: *"Monoclonal Antibody Development for Carbonic Anhydrase 5B (CA5B) in Renal Cell Carcinoma Diagnosis"*
**作者**: Smith, J., et al.
**摘要**: 本研究开发了一种靶向CA5B的单克隆抗体，验证了其在肾细胞癌组织中的特异性结合能力。免疫组化结果显示，CA5B在肿瘤微环境中高表达，提示其作为肾癌诊断标志物的潜力。
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2. **文献名称**: *"CA5B Expression and Antibody-Based Detection in Mitochondrial Disorders"*
**作者**: Lee, H., et al.
**摘要**: 通过制备多克隆抗体，作者分析了CA5B在线粒体功能障碍相关疾病中的表达变化。结果发现CA5B在患者肌肉活检样本中显著下调，表明其可能参与线粒体代谢调控。
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3. **文献名称**: *"Validation of a High-Sensitivity CA5B Antibody for Western Blot and ELISA Applications"*
**作者**: Garcia, R., et al.
**摘要**: 该研究报道了一种高灵敏度的CA5B兔源多克隆抗体的开发与验证。实验证明该抗体在Western blot和ELISA中具有低交叉反应性，适用于定量检测血清及细胞裂解液中的CA5B水平。
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如需真实文献，建议通过PubMed或Google Scholar检索关键词“CA5B antibody”或“Carbonic Anhydrase 5B antibody”获取最新研究。
The CA5B antibody targets carbonic anhydrase 5B (CA-VB), a mitochondrial enzyme belonging to the carbonic anhydrase (CA) family, which catalyzes the reversible hydration of carbon dioxide to bicarbonate and protons. CA5B plays a critical role in pH regulation, mitochondrial metabolism, and biosynthetic processes such as gluconeogenesis and ureagenesis. Unlike cytosolic CA isoforms, CA5B is primarily expressed in metabolically active tissues, including the liver, kidneys, and pancreas, where it supports energy production and acid-base homeostasis.
Research on CA5B has gained traction due to its potential involvement in metabolic disorders and cancer. Dysregulation of CA5B has been linked to mitochondrial dysfunction, and its overexpression in certain tumors suggests a role in cancer cell adaptation to hypoxic conditions. CA5B antibodies are essential tools for detecting protein expression, studying subcellular localization, and exploring its interaction networks in disease models.
Commercially available CA5B antibodies are typically developed in rabbits or mice using immunogenic peptide sequences. They are validated for applications like Western blotting, immunohistochemistry, and immunofluorescence. However, specificity challenges may arise due to homology with other CA isoforms, necessitating careful validation. Current studies focus on CA5B’s therapeutic potential, particularly in modulating metabolic pathways in cancer or metabolic syndromes, making it a subject of growing interest in biomedical research.
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