| WB | DB: 1/500 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Integrin-linked protein kinase, 59 kDa serine/threonine-protein kinase, ILK-1, ILK-2, p59ILK, ILK, ILK1, ILK2 |
| Entrez GeneID | 3611 |
| WB Predicted band size | 51.4kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This ILK Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S343 of human ILK. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于Phospho-ILK(S343)抗体的参考文献示例(内容为虚构，仅供参考)：
1. **文献名称**: *"Phosphorylation of ILK at Serine 343 promotes tumor metastasis via AKT activation"*
**作者**: Li et al.
**摘要**: 研究通过Phospho-ILK(S343)抗体发现，ILK在S343位点的磷酸化通过激活AKT信号通路增强癌细胞侵袭能力，为靶向肿瘤转移提供了新靶点。
2. **文献名称**: *"Role of ILK Ser343 phosphorylation in cardiac fibrosis"*
**作者**: Zhang et al.
**摘要**: 利用Phospho-ILK(S343)特异性抗体证实，ILK的S343磷酸化通过调控TGF-β信号加剧心肌纤维化，提示其在心血管疾病中的病理作用。
3. **文献名称**: *"Phospho-ILK(S343) antibody validates ILK as a biomarker in colorectal cancer"*
**作者**: Wang et al.
**摘要**: 该研究通过免疫组化分析显示，Phospho-ILK(S343)在结直肠癌组织中高表达，且与患者预后不良相关，证明其作为临床标志物的潜力。
4. **文献名称**: *"ILK phosphorylation at Ser343 mediates cell-matrix adhesion dynamics"*
**作者**: Smith et al.
**摘要**: 使用Phospho-ILK(S343)抗体揭示，S343位点磷酸化调控整合素介导的细胞黏附和迁移，影响胚胎发育过程中的细胞运动。
(注：以上文献及作者为示例虚构，实际引用需查询真实数据库如PubMed、Google Scholar等。)
Phospho-ILK(S343) antibody is a specialized tool used to detect the phosphorylated form of Integrin-Linked Kinase (ILK) at serine residue 343. ILK is a multifunctional adaptor protein critical for regulating cell-matrix interactions, survival, proliferation, and migration by bridging integrin receptors with downstream signaling pathways. Phosphorylation at Ser343 is a key post-translational modification that modulates ILK’s activity, influencing its interaction with binding partners like PINCH and parvin, which are essential for cytoskeletal organization and focal adhesion dynamics. This phosphorylation event is often associated with activation of signaling cascades such as the PI3K/Akt and Wnt pathways, which play roles in cancer progression, fibrosis, and cardiovascular diseases.
The Phospho-ILK(S343) antibody is widely utilized in research to investigate ILK’s regulatory mechanisms under physiological or pathological conditions, including tumor metastasis, tissue remodeling, and inflammation. It is commonly applied in techniques like Western blotting, immunofluorescence, and immunohistochemistry to assess ILK activation status in cell lines or tissue samples. Researchers also use it to explore therapeutic targeting of ILK-related pathways, as dysregulated ILK phosphorylation is linked to disease aggressiveness and drug resistance. Proper validation, including knockout controls and peptide competition assays, is recommended to ensure specificity, given the antibody’s reliance on precise phosphorylation-dependent epitope recognition.
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