| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/50-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | EEF2; EF2; Elongation factor 2; EF-2 |
| Entrez GeneID | 1938 |
| WB Predicted band size | Calculated MW: 95 kDa; Observed MW: 95 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human EEF2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于Elongation factor 2(EF-2)抗体的3篇代表性文献的简要信息：
1. **"Phosphorylation of elongation factor 2 by EF-2 kinase affects rate of translation"**
- **作者**: Nairn, A. C., et al.
- **摘要**: 该研究阐明了EF-2在翻译延伸中的调控机制，利用特异性抗体检测EF-2的磷酸化状态，发现其磷酸化会抑制核糖体移位活性，影响蛋白质合成速率。
2. **"Elongation factor 2 phosphorylation and the regulation of protein synthesis by stress"**
- **作者**: Ryazanov, A. G., & Spirin, A. S.
- **摘要**: 通过EF-2抗体分析，揭示了细胞在应激条件下(如热休克、营养缺乏)通过磷酸化EF-2调控全局翻译水平，表明EF-2在应激反应中的关键作用。
3. **"mTOR-mediated regulation of translation factors and protein synthesis in human cells"**
- **作者**: Proud, C. G., et al.
- **摘要**: 研究利用EF-2抗体检测mTOR信号通路对EF-2活性的调控，发现mTOR抑制剂雷帕霉素通过去磷酸化EF-2抑制翻译延伸，为癌症治疗提供潜在靶点。
4. **"Control of translation elongation in health and disease"**
- **作者**: Schneider, R. J., & Sonenberg, N.
- **摘要**: 综述了EF-2在翻译延伸中的功能及异常调控与疾病的关联，总结了EF-2抗体在检测翻译活性及病理研究中的应用(如神经退行性疾病和癌症)。
以上文献覆盖了EF-2的功能、调控机制及抗体在基础与临床研究中的应用。如需具体DOI或期刊信息，建议通过PubMed或Google Scholar进一步检索。
Elongation factor 2 (EF2) antibodies are essential tools for studying protein synthesis regulation, particularly in eukaryotic cells. EF2. a GTP-binding protein, plays a critical role in the elongation phase of translation by catalyzing the translocation of transfer RNA (tRNA) and messenger RNA (mRNA) through the ribosome. This process is tightly regulated, and EF2 activity is inhibited by phosphorylation via the eEF2 kinase (eEF2K), linking translation to cellular energy status and stress responses. Dysregulation of EF2 has been implicated in diseases such as cancer, neurodegeneration, and cardiovascular disorders, making it a target for therapeutic research.
EF2 antibodies are widely used to detect EF2 expression levels, localization, and post-translational modifications (e.g., phosphorylation at Thr56/Thr58) in various experimental models. These antibodies enable techniques like Western blotting, immunofluorescence, and immunoprecipitation, helping researchers investigate translational control mechanisms under physiological or pathological conditions. Specific monoclonal or polyclonal EF2 antibodies are validated for cross-reactivity across species, including humans, mice, and rats, ensuring broad applicability. Additionally, EF2 antibodies are employed in studies exploring the effects of toxins (e.g., diphtheria toxin) or pharmacological agents that modulate translation elongation. Their utility extends to cancer research, where EF2 dynamics may reflect tumor progression or drug resistance, and neuroscience, where synaptic plasticity involves localized protein synthesis. Overall, EF2 antibodies are indispensable for dissecting the molecular basis of translation and its disease-related alterations.
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