| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/50-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | DDX5; G17P1; HELR; HLR1; Probable ATP-dependent RNA helicase DDX5; DEAD box protein 5; RNA helicase p68 |
| Entrez GeneID | 1655 |
| WB Predicted band size | Calculated MW: 69 kDa; Observed MW: 69 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human DDX5 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于DDX5抗体的3篇示例文献(内容为虚构，仅作格式参考)：
1. **文献名称**: "DDX5/p68 RNA helicase as a biomarker in colorectal cancer progression"
**作者**: Smith J, et al.
**摘要**: 本研究通过DDX5特异性抗体检测结直肠癌组织中DDX5蛋白的表达水平，发现其在肿瘤侵袭前沿显著过表达，并与患者预后不良相关。免疫组化与Western blot分析表明，DDX5可能通过调控EMT通路促进肿瘤转移。
2. **文献名称**: "DDX5 regulates pluripotency in embryonic stem cells via mRNA splicing"
**作者**: Lee S, et al.
**摘要**: 利用DDX5抗体进行染色质免疫沉淀(ChIP)和RNA干扰实验，揭示DDX5通过结合特定mRNA前体并调控剪接过程，维持胚胎干细胞多能性。敲低DDX5导致分化相关基因异常剪接。
3. **文献名称**: "DDX5 interacts with HCV core protein to promote viral replication"
**作者**: Tanaka K, et al.
**摘要**: 通过免疫共沉淀(Co-IP)结合DDX5抗体，证实DDX5与丙型肝炎病毒(HCV)核心蛋白直接结合，促进病毒RNA复制。抑制DDX5表达可显著降低病毒载量，提示其作为抗病毒靶点的潜力。
(注：以上文献信息为模拟生成，实际研究中请以真实数据库如PubMed为准。)
The DDX5 antibody is a crucial tool for studying the DEAD-box RNA helicase DDX5. also known as p68. DDX5 belongs to the DEAD-box protein family, characterized by conserved Asp-Glu-Ala-Asp (DEAD) motifs, and plays diverse roles in RNA metabolism. It is involved in transcription, pre-mRNA splicing, ribosome biogenesis, and RNA processing. DDX5 interacts with key regulators like β-catenin, p53. and estrogen receptor-alpha, linking it to cellular processes such as cell cycle progression, viral replication, and cancer development. Its dysregulation is observed in cancers, including breast and colorectal tumors, where it may act as an oncogene or tumor suppressor depending on context.
DDX5 antibodies are widely used in research to detect protein expression, localization, and interactions via techniques like Western blotting, immunoprecipitation, and immunofluorescence. They help elucidate DDX5's roles in gene expression regulation, RNA homeostasis, and disease mechanisms. Commercially available DDX5 antibodies are often raised against specific epitopes, with clones like D12F12 or 3F7 commonly used. Validation includes testing in knockdown models or DDX5-deficient cells to confirm specificity. Researchers also employ these antibodies to explore DDX5's post-translational modifications, such as phosphorylation during mitosis, and its involvement in liquid-liquid phase separation. As DDX5 gains attention as a potential cancer biomarker or therapeutic target, its antibodies remain vital for mechanistic studies and diagnostic development.
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