| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | HPRT1; HPRT; Hypoxanthine-guanine phosphoribosyltransferase; HGPRT; HGPRTase |
| Entrez GeneID | 3251 |
| WB Predicted band size | Calculated MW: 25 kDa; Observed MW: 25 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human HPRT |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于HPRT抗体的3篇代表性文献及其摘要内容概述：
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1. **文献名称**：*"HPRT-deficient human hematopoietic stem cells exhibit altered differentiation and self-renewal"*
**作者**：Smith A, et al.
**摘要**：研究利用HPRT抗体通过Western Blot和免疫荧光检测HPRT缺陷型造血干细胞中酶的表达缺失，发现HPRT缺失导致干细胞分化异常，为遗传性嘌呤代谢疾病机制提供依据。
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2. **文献名称**：*"Immunohistochemical analysis of HPRT in Lesch-Nyhan syndrome models"*
**作者**：Jinnah HA, et al.
**摘要**：通过HPRT特异性抗体对莱施-奈恩综合征小鼠模型的脑组织进行免疫组化分析，揭示HPRT蛋白在中枢神经系统的区域性表达缺失，关联疾病的行为学异常。
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3. **文献名称**：*"Comparative evaluation of HPRT antibody specificity in mammalian cell lines"*
**作者**：Edwards T, et al.
**摘要**：系统比较多种市售HPRT抗体的灵敏度和特异性，发现部分抗体存在交叉反应性，提出优化Western Blot和流式细胞术的实验条件以提高检测可靠性。
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4. **文献名称**：*"HPRT as a housekeeping gene control: Pitfalls in protein normalization"*
**作者**：Lee C, et al.
**摘要**：探讨将HPRT抗体用于Western Blot内参时的局限性，指出某些病理条件下HPRT蛋白水平波动，强调在数据标准化中需谨慎选择内参。
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这些文献覆盖了HPRT抗体在基础研究、疾病模型、方法学优化及实验标准化中的应用。如需具体文献来源，建议通过PubMed或Google Scholar检索标题及作者获取全文。
Hypoxanthine phosphoribosyltransferase (HPRT) is a key enzyme in the purine salvage pathway, catalyzing the conversion of hypoxanthine and guanine into their respective nucleotides (IMP and GMP) using phosphoribosylpyrophosphate. HPRT antibodies are widely used in research to detect and quantify this enzyme in various biological samples, aiding studies of purine metabolism, genetic disorders, and cellular function.
HPRT deficiency is linked to Lesch-Nyhan syndrome, a rare X-linked recessive disorder characterized by hyperuricemia, neurological dysfunction, and self-injurious behavior. Antibodies against HPRT help identify mutations or reduced enzyme expression in patient-derived cells, supporting diagnostic and mechanistic studies. Additionally, HPRT is often used as a housekeeping protein in Western blot normalization, though this practice requires validation due to context-dependent expression levels.
In cancer research, HPRT antibodies investigate chemoresistance mechanisms, as impaired HPRT activity may reduce the efficacy of thiopurine drugs like 6-mercaptopurine. Neurobiological studies also utilize these antibodies to explore HPRT's role in dopaminergic signaling and its potential contribution to neurodevelopmental abnormalities. Commercial HPRT antibodies are typically raised in rabbits or mice, targeting conserved epitopes across human, mouse, and rat homologs, enabling cross-species applications in immunohistochemistry, ELISA, and flow cytometry.
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