| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Homeobox protein OTX1; Homeobox protein OTX2; Orthodenticle 1; Orthodenticle 2; Orthodenticle homeobox 1; Orthodenticle homeobox 2; Orthodenticle1; Orthodenticle2; otx1; otx2 |
| Entrez GeneID | 5013/5015 |
| WB Predicted band size | Calculated MW: 37 kDa; Observed MW: 32 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human Otx1/2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于OTX1/2抗体的3篇参考文献示例(内容为模拟概括，非真实文献)：
1. **"OTX1 expression in brain tumors: a marker of neuroectodermal differentiation"**
- **作者**: Mallamaci, A. et al.
- **摘要**: 研究通过OTX1抗体进行免疫组化和Western blot分析，发现OTX1在胶质母细胞瘤等神经外胚层肿瘤中高表达，提示其可作为神经分化标志物。
2. **"OTX2 is critical for retinal photoreceptor development"**
- **作者**: Beby, F. & Lamonerie, T.
- **摘要**: 利用OTX2特异性抗体在小鼠视网膜中定位OTX2蛋白，证明其在光感受器细胞分化中的关键作用，基因敲除后导致视网膜发育异常。
3. **"OTX2 regulates pituitary gland development and hormone secretion"**
- **作者**: Dateki, S. et al.
- **摘要**: 通过OTX2抗体免疫染色发现，OTX2在垂体腺瘤中异常表达，调控多种激素分泌基因，提示其与垂体功能紊乱相关。
4. **"Dual roles of OTX1 and OTX2 in embryonic patterning"**
- **作者**: Acampora, D. et al.
- **摘要**: 结合OTX1/2抗体研究小鼠胚胎发育，揭示二者在中脑和后脑区域的空间表达差异，参与早期神经管模式形成。
(注：以上文献名称及内容为示例性概括，具体文献需通过PubMed等平台查询确认。)
OTX1 and OTX2 (Orthodenticle homeobox 1/2) are transcription factors belonging to the homeobox gene family, crucial for embryonic development, particularly in the central nervous system, eyes, and sensory organs. They regulate gene expression by binding to DNA, guiding cell differentiation and tissue patterning. OTX1 is primarily associated with forebrain and cerebral cortex development, as well as the maturation of sensory organs like the inner ear and retina. OTX2 plays a broader role, essential for early head formation, eye morphogenesis, and pituitary gland development. Both proteins are evolutionarily conserved, with homologs in various species, reflecting their fundamental biological roles.
Antibodies targeting OTX1/2 are vital tools in developmental biology and disease research. They enable the detection and localization of these proteins in tissues and cells, aiding studies on their expression patterns and functions. In cancer research, OTX1/2 antibodies help investigate their oncogenic roles, as overexpression of OTX2 is linked to medulloblastoma, retinoblastoma, and other tumors. Commercially available antibodies are validated for techniques like Western blotting, immunohistochemistry, and immunofluorescence. Researchers prioritize antibodies with high specificity, often verified using knockout controls or siRNA-mediated protein depletion. Challenges include cross-reactivity due to structural similarities between OTX1 and OTX2. necessitating careful validation. These reagents continue to support advances in understanding developmental disorders and cancer mechanisms.
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