| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/50-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | TS; TMS; HST422 |
| Entrez GeneID | 7298 |
| WB Predicted band size | Calculated MW: 36 kDa; Observed MW: 36 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human Thymidylate Synthase |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于Thymidylate Synthase(TS)抗体的3篇参考文献示例(文献信息为模拟，仅供参考)：
1. **文献名称**：*"Thymidylate Synthase Expression Predicts Resistance to 5-Fluorouracil-Based Chemotherapy in Colorectal Cancer"*
**作者**：Peters, G.J. et al.
**摘要**：该研究通过免疫组化法检测结直肠癌组织中TS蛋白水平，发现高表达TS的患者对5-FU化疗敏感性显著降低，提示TS抗体可作为预测化疗耐药性的生物标志物。
2. **文献名称**：*"Monoclonal Antibodies Against Human Thymidylate Synthase: Comparative Characterization in Cancer Tissues"*
**作者**：Keyomarsi, K. et al.
**摘要**：研究团队开发了多种TS单克隆抗体，并对比其在石蜡包埋组织中的抗原结合特异性，发现不同表位识别的抗体在肿瘤TS定量检测中存在显著差异，强调抗体选择对结果准确性的影响。
3. **文献名称**：*"Development of a Novel TS Polyclonal Antibody for Functional Studies in Cancer Cell Lines"*
**作者**：Rahman, L. et al.
**摘要**：报道了一种新型TS多克隆抗体的制备与验证，通过Western blot和免疫荧光证实其特异性，并应用于沉默TS基因后的功能研究，证明其在基础研究中的可靠性。
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**注**：以上文献信息为示例性模拟，实际引用时请以真实发表的文献为准。建议通过PubMed或Web of Science搜索关键词“Thymidylate Synthase antibody”获取权威文献。
Thymidylate synthase (TS) is a crucial enzyme in DNA synthesis and repair, catalyzing the conversion of deoxyuridine monophosphate (dUMP) to deoxy thymidine monophosphate (dTMP) using 5.10-methylenetetrahydrofolate as a methyl donor. This reaction is essential for maintaining adequate thymidine pools, particularly in rapidly dividing cells. Due to its role in cell proliferation, TS is a key target for chemotherapeutic agents like 5-fluorouracil (5-FU), which inhibit its activity to disrupt DNA replication in cancer cells.
TS antibodies are immunological tools designed to detect and quantify TS protein levels in biological samples. These antibodies are widely used in research to study TS expression patterns in cancers, assess its correlation with disease prognosis, and evaluate resistance to antifolate therapies. Both monoclonal and polyclonal TS antibodies have been developed, validated for applications such as Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and enzyme-linked immunosorbent assays (ELISA). Species specificity typically includes reactivity with human, mouse, and rat TS isoforms.
In clinical contexts, TS overexpression is often linked to poor therapeutic outcomes and drug resistance, making its detection vital for personalized treatment strategies. Research using TS antibodies has advanced understanding of TS regulation, its interaction with chemotherapeutic agents, and its potential as a biomarker for optimizing cancer therapy. These antibodies remain indispensable in both basic research and translational studies aimed at improving targeted anticancer treatments.
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