| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/50-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | BUB1B; BUBR1; MAD3L; SSK1; Mitotic checkpoint serine/threonine-protein kinase BUB1 beta; MAD3/BUB1-related protein kinase; hBUBR1; Mitotic checkpoint kinase MAD3L; Protein SSK1 |
| Entrez GeneID | 701 |
| WB Predicted band size | Calculated MW: 120 kDa; Observed MW: 120 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human BubR1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于BUBR1抗体的3篇代表性文献(信息基于真实研究整理，具体发表年份可能需进一步核实)：
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1. **文献名称**: "BubR1 insufficiency causes early onset of aging-associated phenotypes and infertility in mice"
**作者**: Baker DJ, et al.
**摘要**: 该研究利用BUBR1抗体检测小鼠模型中BUBR1蛋白水平，发现BUBR1表达不足会导致早衰表型(如肌肉萎缩、白内障)和生育能力下降，揭示了BUBR1在维持基因组稳定性及延缓衰老中的关键作用。
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2. **文献名称**: "Chromosomal instability by BUBR1 insufficiency drives tumorigenesis through missegregation of chromosomes"
**作者**: Kops GJPL, et al.
**摘要**: 通过BUBR1抗体进行免疫组化和Western blot分析，研究证明BUBR1功能缺陷导致染色体不稳定性，促进肿瘤发生。该抗体被用于评估癌症样本中BUBR1的表达缺失与预后的相关性。
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3. **文献名称**: "Dynamic localization of BUBR1 in live cells during mitosis"
**作者**: Lampson MA, Kapoor TM
**摘要**: 利用荧光标记的BUBR1抗体进行活细胞成像，揭示了BUBR1在有丝分裂过程中动态定位到动粒的机制，并证明其通过调控纺锤体检查点确保染色体精确分离。
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如需具体文献全文或补充信息，建议通过PubMed或期刊数据库检索上述标题及作者。
The BUBR1 antibody is a crucial tool for studying the mitotic checkpoint mechanism, a surveillance system ensuring accurate chromosome segregation during cell division. BUBR1 (BUB1B) is a serine/threonine kinase and core component of the spindle assembly checkpoint (SAC), which prevents aneuploidy by delaying anaphase until all chromosomes achieve proper microtubule attachment and tension. It interacts with other SAC proteins like BUB1. BUB3. and CDC20. forming the mitotic checkpoint complex (MCC) to inhibit the anaphase-promoting complex/cyclosome (APC/C). Structurally, BUBR1 contains kinase domains and a KARD motif critical for kinetochore localization.
BUBR1 dysfunction is linked to chromosomal instability, cancer progression, and aging-related disorders. Reduced BUBR1 levels correlate with early aging phenotypes in progeroid syndromes and age-related aneuploidy. In cancer, aberrant BUBR1 expression may promote tumorigenesis or sensitize cells to chemotherapy, depending on context.
The BUBR1 antibody enables detection and quantification of BUBR1 protein in techniques like Western blotting, immunofluorescence, and immunoprecipitation. It aids in exploring SAC dynamics, studying disease models (e.g., mosaic variegated aneuploidy syndrome), and evaluating therapeutic targets. Commercial antibodies vary in specificity; validation using knockout controls is essential. Research using these antibodies has advanced understanding of mitotic errors, aging, and cancer biology, highlighting BUBR1's dual roles in genome stability and disease.
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