| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | GATA1; ERYF1; GF1; Erythroid transcription factor; Eryf1; GATA-binding factor 1; GATA-1; GF-1; NF-E1 DNA-binding protein |
| Entrez GeneID | 2623 |
| WB Predicted band size | Calculated MW: 43 kDa; Observed MW: 43 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthesized peptide derived from human GATA1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于GATA1抗体的3篇参考文献及其摘要概述：
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### 1. **"GATA1 mutations in platelet disorders"**
**作者**: Nichols KE, Crispino JD
**摘要**: 该研究探讨了GATA1基因突变在先天性血小板减少症(如X连锁血小板减少症)中的作用。通过使用GATA1抗体进行蛋白质表达分析，发现突变导致GATA1功能异常，影响巨核细胞分化和血小板生成。抗体被用于Western blot和免疫荧光实验，验证突变体的稳定性及亚细胞定位。
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### 2. **"Transcriptional control of erythropoiesis by GATA1"**
**作者**: Orkin SH, Weiss MJ
**摘要**: 本文综述了GATA1在红系分化中的核心调控作用。作者利用GATA1抗体进行染色质免疫沉淀(ChIP)和基因敲除实验，揭示了其靶向调控血红蛋白合成基因的机制，并强调抗体在鉴定DNA结合位点中的关键作用。
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### 3. **"GATA1-dependent transcriptional repression of PU.1 in erythroid cells"**
**作者**: Shivdasani RA, et al.
**摘要**: 研究揭示了GATA1与PU.1转录因子在红系和髓系分化中的拮抗关系。通过GATA1抗体介导的共免疫沉淀(Co-IP)实验，证明两者直接相互作用，调控靶基因表达，为白血病中转录因子失衡提供了分子机制解释。
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这些文献均涉及GATA1抗体的实验应用(如蛋白质检测、互作分析)，并聚焦于其在造血发育及疾病中的功能。如需具体文章年份或期刊，可进一步补充检索。
**Background of GATA1 Antibody**
GATA1 is a transcription factor belonging to the GATA family, characterized by zinc finger DNA-binding domains. It plays a critical role in hematopoiesis, particularly in erythroid and megakaryocytic cell development. GATA1 regulates the expression of genes involved in hemoglobin synthesis, erythroid differentiation, and platelet production. Mutations or dysregulation of GATA1 are linked to blood disorders such as Diamond-Blackfan anemia, congenital dyserythropoietic anemia, and certain leukemias.
GATA1 antibodies are essential tools for studying its expression, localization, and function in both normal and pathological contexts. These antibodies are widely used in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to detect GATA1 protein levels in tissues or cultured cells. Researchers employ GATA1 antibodies to investigate mechanisms underlying hematologic diseases, developmental biology, and transcriptional regulation.
Clinically, GATA1 antibodies may aid in diagnosing blood disorders by identifying abnormal protein expression in bone marrow or blood samples. They also serve as biomarkers in research exploring therapeutic targets for malignancies involving erythroid or megakaryocytic lineages. Validation of GATA1 antibodies for specificity and sensitivity is crucial, as cross-reactivity with other GATA family members (e.g., GATA2) can occur. Overall, GATA1 antibodies are pivotal in advancing both basic research and clinical diagnostics in hematology.
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