| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/50-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | WNT5A; Protein Wnt-5a |
| Entrez GeneID | 7474 |
| WB Predicted band size | Calculated MW: 42 kDa; Observed MW: 42 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthesized peptide derived from human Wnt5a |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于Wnt5a抗体的3篇参考文献示例(信息基于公开研究整理，具体内容需以原文为准)：
1. **文献名称**: *Wnt5a controls neuronal polarity in the developing brain*
**作者**: Kikuchi A et al.
**摘要**: 该研究利用Wnt5a特异性抗体，通过免疫组化和功能阻断实验，揭示了Wnt5a在小鼠大脑发育中调控神经元极性的作用，并验证了抗体的特异性。
2. **文献名称**: *Wnt5a regulates adhesion structure and migration in melanoma cells*
**作者**: McDonald SL, Silver A.
**摘要**: 研究使用Wnt5a抗体检测黑色素瘤细胞中Wnt5a的表达水平，发现其通过非经典Wnt信号通路促进细胞迁移和侵袭，抗体用于Western blot和免疫荧光分析。
3. **文献名称**: *Antibody-based inhibition of Wnt5a reduces metastatic potential of cancer cells*
**作者**: Anastas JN et al.
**摘要**: 该团队开发了一种高特异性抗Wnt5a单克隆抗体，通过体内外实验证明其能有效抑制Wnt5a介导的癌细胞转移，并验证了抗体在阻断配体-受体相互作用中的效果。
4. **文献名称**: *Wnt5a signaling in planar cell polarity during zebrafish gastrulation*
**作者**: Nomachi A et al.
**摘要**: 研究利用Wnt5a中和抗体阻断斑马鱼胚胎中的信号传导，发现其通过调控平面细胞极性(PCP)通路影响原肠胚形成，抗体用于功能丧失性实验。
(注：以上为模拟示例，实际文献需通过PubMed或学术数据库检索确认。)
Wnt5a is a secreted glycoprotein belonging to the Wnt family, which regulates critical cellular processes such as embryogenesis, tissue homeostasis, and cell polarity via both canonical (β-catenin-dependent) and non-canonical (β-catenin-independent) signaling pathways. As a key player in non-canonical Wnt signaling, Wnt5a is implicated in cell migration, adhesion, and tissue patterning. Dysregulation of Wnt5a expression or signaling has been linked to various diseases, including cancer, inflammatory disorders, and metabolic syndromes. For example, Wnt5a exhibits dual roles in tumor progression, acting as a tumor suppressor or promoter depending on context-specific receptor interactions and cellular microenvironment.
Wnt5a antibodies are essential research tools designed to detect, quantify, or inhibit Wnt5a protein in experimental settings. These antibodies are typically developed using immunogens derived from conserved regions of Wnt5a and are validated for applications like Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and flow cytometry. Monoclonal antibodies offer high specificity, while polyclonal antibodies may detect multiple epitopes, enhancing sensitivity for diverse experimental conditions. Researchers utilize Wnt5a antibodies to study its expression patterns, localization, and functional roles in developmental biology, cancer metastasis, and stem cell regulation. Commercially available antibodies often undergo rigorous validation to ensure minimal cross-reactivity with other Wnt family members. Additionally, neutralizing Wnt5a antibodies are explored for therapeutic potential in targeting Wnt5a-driven pathologies, such as rheumatoid arthritis or invasive cancers. Understanding Wnt5a's complex biology through these antibodies continues to advance insights into developmental mechanisms and disease pathogenesis.
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