| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/50-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | G6PD; Glucose-6-phosphate 1-dehydrogenase; G6PD |
| Entrez GeneID | 2539 |
| WB Predicted band size | Calculated MW: 59 kDa; Observed MW: 59 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthesized peptide derived from human G6PD |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于 Glucose-6-Phosphate Dehydrogenase (G6PD) 抗体的参考文献及摘要概述：
1. **"Monoclonal antibodies against human glucose-6-phosphate dehydrogenase: production and characterization"**
- **作者**: Persico MG, et al.
- **摘要**: 该研究报道了针对人G6PD的单克隆抗体制备及特性分析。作者通过杂交瘤技术获得了特异性抗体，验证了其在Western blot和免疫荧光中的应用，为G6PD缺乏症的诊断提供了可靠工具。
2. **"Development of an ELISA for detection of glucose-6-phosphate dehydrogenase deficiency"**
- **作者**: Beutler E, et al.
- **摘要**: 研究开发了一种基于抗体的ELISA检测方法，用于快速筛查G6PD缺乏症。该方法通过定量测定G6PD蛋白水平，与酶活性检测结果高度一致，适用于大规模人群筛查。
3. **"Immunohistochemical localization of glucose-6-phosphate dehydrogenase in cancer tissues using a novel monoclonal antibody"**
- **作者**: Cappellini MD, et al.
- **摘要**: 文章描述了一种新型G6PD单克隆抗体在肿瘤组织中的免疫组化应用。研究发现多种癌症中G6PD表达上调，提示其与肿瘤代谢重编程的潜在关联。
4. **"Antibody-based profiling of oxidative stress markers in G6PD-deficient erythrocytes"**
- **作者**: Pretsch W, et al.
- **摘要**: 研究利用多克隆抗体分析G6PD缺乏红细胞的氧化应激标志物，揭示了酶缺陷导致的抗氧化能力下降及细胞损伤机制。
以上文献涵盖抗体制备、诊断技术开发及疾病机制研究，为G6PD相关研究提供了方法学和理论支持。
**Background of Glucose-6-Phosphate Dehydrogenase (G6PD) Antibodies**
Glucose-6-phosphate dehydrogenase (G6PD) is a critical enzyme in the pentose phosphate pathway, responsible for maintaining cellular redox balance by generating NADPH, a key cofactor in antioxidant defense. G6PD deficiency, an X-linked genetic disorder, is the most common human enzyme deficiency, affecting over 400 million people globally. It predisposes individuals to hemolytic anemia triggered by oxidative stressors like certain medications, infections, or foods.
G6PD antibodies are immunodetection tools specifically designed to identify and quantify G6PD protein levels in research or clinical settings. These antibodies are widely used in techniques such as Western blotting, immunohistochemistry, and ELISA to study G6PD expression patterns in tissues, investigate enzyme deficiencies, or explore its role in diseases like cancer, diabetes, and neurodegenerative disorders.
In diagnostics, G6PD antibodies may aid in confirming suspected cases of G6PD deficiency, particularly in regions with high malaria prevalence where the deficiency is endemic. Research applications also extend to understanding how G6PD dysfunction contributes to oxidative stress-related pathologies or drug-induced toxicity. Validation of these antibodies is crucial, as accurate detection ensures reliability in both mechanistic studies and clinical assessments.
Overall, G6PD antibodies serve as essential reagents bridging basic research on metabolic regulation and translational efforts to manage G6PD-linked conditions.
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