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Rabbit Monoclonal PolycombProteinSUZ12 Antibody

  • 中文名： Polycomb Protein SUZ12抗体
  • 别    名： SUZ12; CHET9; JJAZ1; KIAA0160; Polycomb protein SUZ12; Chromatin precipitated E2F target 9 protein; ChET 9 protein; Joined to JAZF1 protein; Suppressor of zeste 12 protein homolog
货号: IPDX22592
Price： ￥1280
数量：
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验证与应用

应用及物种
WB 1/500-1/1000 Human,Mouse,Rat
IF 1/20 Human,Mouse,Rat
IHC 咨询技术 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesSUZ12; CHET9; JJAZ1; KIAA0160; Polycomb protein SUZ12; Chromatin precipitated E2F target 9 protein; ChET 9 protein; Joined to JAZF1 protein; Suppressor of zeste 12 protein homolog
Entrez GeneID23512
WB Predicted band sizeCalculated MW: 83 kDa; Observed MW: 83 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman,Mouse
ImmunogenA synthetic peptide of human SUZ12
FormulationPurified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol.

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参考文献

以下是3篇关于Polycomb蛋白SUZ12抗体的参考文献，按文献内容概括：

1. **"Control of developmental regulators by Polycomb in human embryonic stem cells"**

*作者*：Lee TI, et al. (2006)

*摘要*：该研究利用SUZ12抗体进行ChIP-seq分析，揭示了SUZ12在人类胚胎干细胞中与Polycomb抑制复合物PRC2的结合位点，证明其通过H3K27me3修饰调控发育相关基因的沉默。

2. **"Suz12 is essential for mouse development and for EZH2 histone methyltransferase activity"**

*作者*：Pasini D, et al. (2004)

*摘要*：通过生成Suz12条件性敲除小鼠，结合SUZ12抗体的免疫沉淀和Western blot实验，证实SUZ12是PRC2复合物功能的核心组分，其缺失导致EZH2的组蛋白甲基转移酶活性丧失。

3. **"The Polycomb group protein Suz12 is required for embryonic stem cell differentiation"**

*作者*：Montgomery ND, et al. (2005)

*摘要*：使用SUZ12抗体进行免疫荧光和RNA干扰实验，证明SUZ12在小鼠胚胎干细胞分化中不可或缺，其缺失导致多能性基因持续表达和分化阻滞，并通过Western blot验证抗体特异性。

4. **"Polycomb complexes repress developmental regulators in murine embryonic stem cells"**

*作者*：Boyer LA, et al. (2006)

*摘要*：通过SUZ12抗体的ChIP-chip技术，绘制了PRC2在小鼠胚胎干细胞中的靶基因图谱，发现其优先结合发育调控基因，并依赖SUZ12维持H3K27me3修饰和基因沉默状态。

以上文献均涉及SUZ12抗体在机制研究中的应用(如ChIP、Western blot、功能缺失实验)，并验证了其特异性，可作为SUZ12相关研究的经典参考。

背景信息

The Polycomb protein SUZ12 (Suppressor of Zeste 12 homolog) is a core component of the Polycomb Repressive Complex 2 (PRC2), a critical epigenetic regulator that mediates gene silencing through trimethylation of histone H3 on lysine 27 (H3K27me3). SUZ12 plays a structural and functional role in stabilizing the PRC2 complex, which also includes EED, EZH2 (or EZH1), and RBBP4/7. Antibodies targeting SUZ12 are widely used in research to study PRC2-mediated chromatin remodeling, cellular differentiation, and developmental processes. They enable the detection of SUZ12 expression levels, localization, and interaction partners via techniques like Western blotting, immunoprecipitation (IP), chromatin immunoprecipitation (ChIP-seq), and immunofluorescence. Dysregulation of SUZ12 is implicated in cancers, neurodevelopmental disorders, and other diseases, making its antibody a vital tool for exploring PRC2 dysfunction mechanisms. Validation of SUZ12 antibodies typically involves knockout cell lines or siRNA-mediated depletion to confirm specificity. Researchers also use these antibodies to investigate PRC2's role in maintaining stem cell pluripotency, X-chromosome inactivation, and imprinting. Given SUZ12's conserved role across species, its antibodies are applicable in diverse model organisms, aiding cross-species comparisons in epigenetic studies.

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