| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | STAT5 |
| Entrez GeneID | 6777 |
| WB Predicted band size | Calculated MW: 90 kDa; Observed MW: 90 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Recombinant protein of human STAT5b |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于STAT5b抗体的3-4篇参考文献，简要概括了文献名称、作者及摘要内容：
1. **文献名称**：*STAT5b regulates the transcriptional response of T cells to IL-2 and coordinates distinct immune functions*
**作者**：Lin, J.X., et al.
**摘要**：该研究利用STAT5b特异性抗体，揭示了STAT5b在IL-2信号通路中的核心作用，证明其通过调控下游基因转录协调T细胞增殖、存活和免疫抑制功能。
2. **文献名称**：*STAT5b is a critical mediator of GH-induced hepatic gene expression and body growth*
**作者**：Hennighausen, L., et al.
**摘要**：通过STAT5b抗体进行染色质免疫沉淀(ChIP)实验，发现STAT5b是生长激素(GH)信号转导的关键介质，直接影响肝脏基因表达及全身代谢调控。
3. **文献名称**：*Phosphorylated STAT5b as a biomarker for aggressive prostate cancer and therapeutic targeting*
**作者**：Yu, H., et al.
**摘要**：研究利用磷酸化STAT5b抗体检测前列腺癌组织，发现STAT5b的持续激活与肿瘤侵袭性相关，提示其可作为预后标志物及潜在治疗靶点。
4. **文献名称**：*STAT5b-dependent regulation of breast cancer stem cell self-renewal and tumorigenicity*
**作者**：Watson, C.J., et al.
**摘要**：通过STAT5b抗体阻断实验，证实STAT5b信号通路在维持乳腺癌干细胞自我更新和肿瘤发生中的必要性，为靶向治疗提供依据。
这些文献涵盖了STAT5b在免疫、代谢、癌症等领域的分子机制及抗体应用，具体实验方法包括Western blot、免疫组化及ChIP-seq等。如需更详细信息，建议通过PubMed或Web of Science检索DOI或PMID。
**Background of STAT5b Antibody**
The STAT5b (Signal Transducer and Activator of Transcription 5B) antibody is a crucial tool for studying the STAT protein family, which mediates cellular responses to cytokines, growth factors, and hormones. STAT5b, along with its isoform STAT5a, is activated via phosphorylation by receptor-associated kinases (e.g., JAK kinases), leading to dimerization, nuclear translocation, and regulation of target genes involved in proliferation, differentiation, and survival.
STAT5b-specific antibodies are designed to distinguish STAT5b from STAT5a, despite their high sequence homology (~90%). These antibodies enable researchers to investigate isoform-specific functions, as STAT5b has distinct roles in immune regulation, lactation, and growth hormone signaling. For example, STAT5b knockout mice exhibit growth defects and immune dysregulation, highlighting its physiological importance.
In research, STAT5b antibodies are widely used in techniques like Western blotting, immunohistochemistry (IHC), and immunoprecipitation (IP) to detect protein expression, activation (phospho-STAT5b), and localization in tissues or cell lines. They are particularly valuable in cancer studies, as aberrant STAT5b signaling is linked to leukemia, breast cancer, and prostate cancer. Additionally, these antibodies aid in exploring STAT5b's role in metabolic disorders and immune pathologies.
Commercial STAT5b antibodies are often validated for specificity and sensitivity, with applications in both basic and translational research, offering insights into disease mechanisms and therapeutic targeting of the JAK-STAT pathway.
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