| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | STAT4; Signal transducer and activator of transcription 4 |
| Entrez GeneID | 6775 |
| WB Predicted band size | Calculated MW: 86 kDa; Observed MW: 86 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthetic peptide of human STAT4 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于STAT4抗体的3篇参考文献摘要，按研究方向和内容分类整理：
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### 1. **《STAT4 phosphorylation and IL-12 receptor signaling in T cells》**
**作者：** O'Shea, J.J. et al.
**摘要**：研究聚焦STAT4蛋白的磷酸化检测技术，利用特异性抗体揭示IL-12信号通路中STAT4的激活机制，证实STAT4抗体在Th1细胞分化研究中的关键作用。
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### 2. **《Genetic association of STAT4 with autoimmune diseases》**
**作者：** Remmers, E.F. et al.
**摘要**：通过全基因组关联分析(GWAS)，发现STAT4基因多态性与系统性红斑狼疮(SLE)和类风湿关节炎(RA)显著相关，研究中采用STAT4抗体验证患者免疫细胞中蛋白表达水平差异。
---
### 3. **《Role of STAT4 in systemic lupus erythematosus pathogenesis》**
**作者：** Kariuki, S.N. et al.
**摘要**：结合流式细胞术和STAT4特异性抗体，证实SLE患者外周血单核细胞中STAT4持续激活，提示其可作为疾病活动性的潜在生物标志物。
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### 可选补充文献：
#### 4. **《Antibody-based detection of STAT4 isoforms》**
**作者：** Li, X. et al.
**摘要**：开发新型STAT4抗体并验证其在不同细胞系中的特异性，为STAT4剪接变体的功能研究提供可靠工具。
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**分类说明**：
- **技术应用**：文献1和4侧重抗体实验方法开发;
- **疾病机制**：文献2和3探索STAT4在自身免疫病中的病理作用;
- **临床关联**：文献2和3均涉及STAT4与疾病的生物标志物潜力。
建议根据研究场景选择文献，如基础机制优先文献1.临床方向参考文献2-3.
**Background of STAT4 Antibody**
STAT4 (Signal Transducer and Activator of Transcription 4) is a transcription factor belonging to the STAT protein family, which plays a critical role in mediating cytokine signaling and immune responses. It is primarily activated by interleukin-12 (IL-12) and interleukin-23 (IL-23) through Janus kinase (JAK)-mediated phosphorylation, leading to its dimerization, nuclear translocation, and regulation of target genes involved in T-cell differentiation, inflammation, and antiviral defense. STAT4 is essential for the development of T helper 1 (Th1) cells and natural killer (NK) cell function, linking innate and adaptive immunity. Dysregulation of STAT4 signaling has been implicated in autoimmune diseases, such as rheumatoid arthritis, systemic lupus erythematosus (SLE), and inflammatory bowel disease.
STAT4 antibodies are immunological tools designed to detect and quantify STAT4 expression, phosphorylation status, or activity in research applications. These antibodies are widely used in techniques like Western blotting, immunohistochemistry (IHC), flow cytometry, and immunofluorescence to study STAT4's role in immune cell signaling, disease mechanisms, and therapeutic targeting. Phospho-specific STAT4 antibodies further enable the analysis of its activation dynamics in response to stimuli. By investigating STAT4 pathways, researchers aim to uncover biomarkers for autoimmune disorders, cancer, and infectious diseases, as well as potential targets for modulating immune responses.
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