| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | STK39; SPAK; STE20/SPS1-related proline-alanine-rich protein kinase; Ste-20-related kinase; DCHT; Serine/threonine-protein kinase 39 |
| Entrez GeneID | 27347 |
| WB Predicted band size | Calculated MW: 59 kDa; Observed MW: 65 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthetic peptide of human SPAK |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于SPAK抗体的3篇参考文献及其摘要概括：
1. **"Characterization of SPAK kinase activation and phosphorylation of the Na-K-2Cl cotransporter"**
*作者：Richardson C, Alessi DR*
摘要：该研究利用SPAK特异性抗体，揭示了SPAK激酶在磷酸化Na-K-2Cl协同转运蛋白(NKCC1/2)中的关键作用，并证实其在细胞离子稳态调节中的核心地位。
2. **"The WNK-SPAK/OSR1 signaling pathway in electrolyte homeostasis and blood pressure regulation"**
*作者：Moriguchi T, Urushiyama S*
摘要：通过SPAK抗体的免疫沉淀实验，文章阐明了WNK激酶通过激活SPAK/OSR1通路调控肾脏离子通道的机制，并探讨了该通路与遗传性高血压的关联。
3. **"SPAK kinase is a substrate and target of PKCθ in T-cell receptor signaling"**
*作者：Zhao Y, Zhang X*
摘要：研究使用SPAK磷酸化特异性抗体，揭示了T细胞受体信号中PKCθ对SPAK的调控作用，提示SPAK在免疫细胞活化及炎症反应中的潜在功能。
注：以上文献为示例，实际引用需核对真实出版物信息。如需具体文献，建议通过PubMed/Google Scholar以“SPAK antibody”、“SPAK kinase signaling”为关键词检索近年高被引论文。
The SPAK (STE20/SPS1-related proline-alanine-rich kinase) antibody is a critical tool for studying the SPAK protein, a serine/threonine kinase belonging to the STE20 kinase family. SPAK plays a key role in cellular ion homeostasis by regulating cation-chloride cotransporters (e.g., NKCC1. NKCC2. KCC1-3) through phosphorylation. It is activated by upstream kinases, such as WNK1-4 (With-No-Lysine kinases), in response to osmotic stress or electrolyte imbalances. SPAK interacts with OSR1 (oxidative stress-responsive kinase 1), forming a signaling cascade that modulates ion transport in the kidneys, neurons, and other tissues. Dysregulation of SPAK activity is linked to hypertension, neurological disorders, and electrolyte abnormalities.
The SPAK antibody enables researchers to detect SPAK expression, localization, and phosphorylation status in various experimental models. It is widely used in techniques like Western blotting, immunohistochemistry, and immunoprecipitation to investigate SPAK’s involvement in cellular signaling pathways, drug responses, or disease mechanisms. Specific SPAK antibodies targeting distinct epitopes (e.g., phosphorylated Thr233/Ser373) help dissect its activation dynamics. Development of SPAK inhibitors for hypertension treatment has further driven demand for reliable antibodies to validate target engagement and therapeutic efficacy. Overall, SPAK antibodies are indispensable for advancing research on ion regulation, cellular stress responses, and related pathologies.
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