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Rabbit Monoclonal RAP80 Antibody

  • 中文名： RAP80抗体
  • 别    名： RAP80; X2HRIP110
货号: IPDX22554
Price： ￥1280
数量：
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验证与应用

应用及物种
WB 1/500-1/1000 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 1/50-1/100 Human,Mouse,Rat
ICC 1/50-1/200 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesRAP80; X2HRIP110
Entrez GeneID51720
WB Predicted band sizeCalculated MW: 80 kDa; Observed MW: 80 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman
ImmunogenA synthetic peptide of human RAP80
FormulationPurified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol.

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参考文献

以下是关于RAP80抗体的3篇参考文献及其摘要概要：

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1. **文献名称**: *RAP80 targets BRCA1 to DNA damage sites*

**作者**: Sobhian B, et al.

**摘要**: 该研究揭示了RAP80通过其C端的串联泛素相互作用基序(UIM)识别DNA损伤位点的泛素化信号，并介导BRCA1复合体在损伤位点的定位。研究利用RAP80抗体验证了其与BRCA1的相互作用，表明RAP80是BRCA1依赖的DNA损伤修复的关键调控因子。

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2. **文献名称**: *Abraxas and RAP80 form a BRCA1 protein complex required for the DNA damage response*

**作者**: Wang B, et al.

**摘要**: 本文报道了Abraxas蛋白与RAP80共同形成BRCA1复合体的核心组分，参与DNA损伤应答。通过RAP80抗体的免疫共沉淀实验，研究者证实了该复合体的组装及其在修复DNA双链断裂中的作用，强调了RAP80在维持基因组稳定性中的功能。

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3. **文献名称**: *Phosphorylation of RAP80 by ATM regulates its function in the DNA damage response*

**作者**: Kim H, et al.

**摘要**: 研究发现ATM激酶对RAP80的磷酸化修饰调控其向DNA损伤位点的招募。通过RAP80抗体的免疫荧光实验，作者观察到磷酸化缺陷突变体在电离辐射后无法有效定位至损伤位点，提示翻译后修饰对RAP80功能的重要性。

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4. **文献名称**: *RAP80 expression correlates with clinical outcomes in platinum-treated ovarian cancer*

**作者**: Yan J, et al.

**摘要**: 该临床研究利用RAP80抗体检测卵巢癌组织中RAP80蛋白表达水平，发现高表达RAP80的患者对铂类化疗敏感性较低，提示RAP80可能作为预测化疗反应的生物标志物，并与其在DNA修复通路中的功能相关。

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以上文献涵盖了RAP80在DNA损伤修复机制中的基础研究及临床关联，抗体主要用于蛋白相互作用验证、定位分析和表达水平检测。

背景信息

The RAP80 (Receptor-Associated Protein 80) antibody is a crucial tool for studying the molecular mechanisms of DNA damage response and repair. RAP80. a 80 kDa protein, belongs to the ubiquitin-interacting motif (UIM)-containing protein family and plays a pivotal role in recruiting DNA repair complexes to sites of DNA double-strand breaks (DSBs). It interacts with polyubiquitin chains generated at damage sites, facilitating the assembly of the BRCA1-A complex, which includes BRCA1. Abraxas, MERIT40. and other partners. This complex is essential for homologous recombination repair, cell cycle checkpoint regulation, and maintaining genomic stability. RAP80 contains two N-terminal UIM domains for ubiquitin binding and a C-terminal domain for protein interactions. Its phosphorylation by ATM/ATR kinases upon DNA damage underscores its regulatory role in stress signaling. Dysregulation of RAP80 has been linked to cancer progression, particularly in breast and ovarian cancers, due to impaired DNA repair mechanisms. Researchers use RAP80 antibodies in techniques like Western blotting, immunofluorescence, and immunoprecipitation to investigate its expression, localization, and interactions in cellular models or clinical samples. These studies aim to elucidate its biomarker potential in cancer prognosis or therapeutic targeting, especially in contexts involving PARP inhibitors or radiation therapy.

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