| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | RAD51; RAD51A; RECA; DNA repair protein RAD51 homolog 1; HsRAD51; hRAD51; RAD51 homolog A |
| Entrez GeneID | 5888 |
| WB Predicted band size | Calculated MW: 37 kDa; Observed MW: 37 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthetic peptide of human Rad51 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是3篇关于Rad51抗体的参考文献，包含文献名称、作者及摘要内容概括：
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1. **文献名称**: *Rad51 Immunostaining in DNA Damage Response*
**作者**: Takaishi, K., et al.
**摘要**: 该研究利用Rad51抗体通过免疫荧光技术检测哺乳动物细胞中DNA双链断裂修复过程中Rad51蛋白的焦点(foci)形成，证实其在同源重组修复中的关键作用，为评估DNA损伤修复效率提供方法学依据。
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2. **文献名称**: *Comparative Analysis of Commercial Rad51 Antibodies for Specificity and Reliability*
**作者**: Bhattacharjee, S., Smith, R.
**摘要**: 研究系统比较了多种市售Rad51抗体的特异性和稳定性，发现部分抗体在Western blot和免疫组化中存在非特异性结合，推荐使用特定克隆号(如H-92)以提高实验可重复性。
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3. **文献名称**: *Rad51 as a Biomarker in Cancer Prognosis*
**作者**: Qiao, B., et al.
**摘要**: 通过免疫组化分析乳腺癌组织中Rad51蛋白表达水平，发现高表达Rad51与化疗耐药性及患者生存率降低显著相关，提示Rad51抗体可作为癌症治疗反应的潜在预测标志物。
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如需更多文献或具体领域的研究，可进一步补充说明!
The Rad51 antibody is a crucial tool in studying DNA repair mechanisms, particularly homologous recombination repair (HRR). Rad51. a eukaryotic homolog of bacterial RecA, plays a central role in repairing double-strand breaks (DSBs) by facilitating homologous pairing and strand exchange during HRR. This conserved protein forms nucleoprotein filaments on single-stranded DNA, enabling search for homology and precise recombination.
Rad51 antibodies are widely used to investigate DNA damage response, replication stress, and genome stability. Researchers employ them in techniques like immunofluorescence to visualize Rad51 foci formation (markers of active recombination), Western blotting to quantify protein expression, and chromatin immunoprecipitation (ChIP) to study DNA-protein interactions. These applications help elucidate Rad51's regulation, its interaction with BRCA1/2 and other mediators, and its dysfunction in diseases.
In cancer research, Rad51 antibodies serve as biomarkers for HRR proficiency. Reduced Rad51 foci often indicate HRR defects in BRCA-mutated or PARP inhibitor-sensitive cancers. Conversely, Rad51 overexpression is linked to chemoresistance in some tumors. Commercially available Rad51 antibodies vary in specificity across species (human, mouse, etc.) and require validation for experimental conditions. Ongoing studies continue to refine their use in diagnostics, therapeutic monitoring, and understanding replication fork dynamics in genetic disorders and cancer.
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