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Rabbit Monoclonal Rad50 Antibody

  • 中文名： Rad50抗体
  • 别    名： RAD50; DNA repair protein RAD50; hRAD50
货号: IPDX22550
Price： ￥1280
数量：
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验证与应用

应用及物种
WB 1/500-1/1000 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 1/50-1/100 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesRAD50; DNA repair protein RAD50; hRAD50
Entrez GeneID10111
WB Predicted band sizeCalculated MW: 154 kDa; Observed MW: 154 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman
ImmunogenRecombinant protein of human Rad50
FormulationPurified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol.

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参考文献

以下是关于Rad50抗体的3篇参考文献及其摘要内容：

1. **"The Mre11 complex and the metabolism of chromosome breaks: the importance of communicating and holding things together"**

- **作者**: Stracker, T.H., Theunissen, J.W., Morales, M., Petrini, J.H.

- **摘要**: 该综述讨论了Mre11复合体(包括Rad50、Mre11和Nbs1)在DNA双链断裂修复中的核心作用，强调了Rad50抗体在解析复合体结构和功能中的应用，例如通过免疫沉淀和免疫荧光验证其亚细胞定位及相互作用。

2. **"Mre11 nuclease activity and CtIP regulate resection at DNA double-strand breaks"**

- **作者**: Buis, J., Wu, Y., Deng, Y., et al.

- **摘要**: 研究利用Rad50抗体在基因敲除小鼠模型中验证Rad50蛋白表达缺失对DNA末端切除的影响，发现Rad50与CtIP协同调控DNA断裂修复的早期步骤，抗体用于Western blot和免疫组化分析。

3. **"Nbs1 promotes ATM-dependent phosphorylation events through the Mre11-Rad50 complex"**

- **作者**: Williams, R.S., Dodson, G.E., Limbo, O., et al.

- **摘要**: 通过Rad50抗体进行免疫共沉淀实验，揭示Rad50在ATM激酶激活中的桥梁作用，研究显示Rad50-Mre11复合体通过构象变化传递DNA损伤信号，抗体在蛋白质相互作用和磷酸化检测中起关键作用。

4. **"DNA double-strand break repair pathway choice is directed by distinct Mre11 nuclease activities"**

- **作者**: Shibata, A., Conrad, S., Birraux, J., et al.

- **摘要**: 该研究使用Rad50抗体进行免疫荧光染色，发现Rad50在不同修复通路选择中的动态定位，表明其核酸酶活性调控同源重组与非同源末端连接的平衡，抗体用于活细胞成像和蛋白质表达定量。

这些文献均通过Rad50抗体在分子机制研究中验证蛋白功能、定位或相互作用，涵盖DNA修复、信号传导及疾病模型等多个方向。

背景信息

Rad50 antibody is a key tool for studying the Rad50 protein, a critical component of the Mre11-Rad50-Nbs1 (MRN) complex involved in DNA damage response and repair. Rad50. an ATPase with structural similarities to structural maintenance of chromosomes (SMC) proteins, plays a central role in detecting and signaling DNA double-strand breaks (DSBs), facilitating homologous recombination repair, and maintaining genomic stability. The MRN complex coordinates DNA end resection, activates ATM kinase signaling, and stabilizes damaged DNA structures. Rad50 antibodies are widely used in research to investigate its expression, localization, and interactions via techniques like Western blotting, immunofluorescence, and immunoprecipitation. These antibodies help elucidate Rad50's role in cancer biology, as dysregulation or mutations in Rad50 are linked to genomic instability, tumorigenesis, and chemotherapy resistance. They also aid in studying inherited disorders like Nijmegen breakage syndrome, where MRN complex dysfunction causes hypersensitivity to ionizing radiation. Commercially available Rad50 antibodies are typically raised in hosts such as rabbits or mice, with validation across species (human, mouse, rat). Specificity is confirmed using knockout cell lines or siRNA knockdown. Researchers rely on these reagents to explore Rad50's structural roles in bridging DNA ends, its ATP-dependent conformational changes, and its collaboration with repair factors like BRCA1/2. Proper antibody validation remains essential due to potential cross-reactivity with other SMC family members.

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