首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠载脂蛋白B-48(ApoB48)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的ApoB48含量。ApoB48 是一种重要靶点。它主要在小肠合成，参与乳糜微粒组装和分泌。研究发现其与脂质代谢密切相关，异常升高可能导致血脂异常等问题。目前科研聚焦于通过抑制 ApoB48 生成或功能，调节脂质代谢，为治疗相关疾病提供新思路。试剂盒检测范围为0.312 μg/mL-20 μg/mL，适用于营养干预实验、代谢性疾病模型研究、药物对肠道脂质吸收影响的评估等科研领域，为探索脂代谢调控机制及心血管疾病相关研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：ApoB48
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：0.312 μg/mL-20 μg/mL
灵敏度：0.078 μg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat ApoB48 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 88 | ||||
| Range % | 84-92 | |||||
| 1:200 | Average % | 96 | ||||
| Range % | 92-102 | |||||
| 1:400 | Average % | 95 | ||||
| Range % | 89-100 | |||||
| 1:800 | Average % | 92 | ||||
| Range % | 89-96 | |||||
回收率：
| The recovery of rat ApoB48 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 98 | 94-102 | ||||
| EDTA plasma (n=4) | 92 | 88-97 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| μg/ml.OD1OD2Average 200.0740.0760.075 100.1200.1260.123 50.1820.1850.184 2.50.2830.2860.285 1.250.4510.4680.460 0.6250.7700.7850.778 0.3121.1221.1621.142 02.3712.3922.382 | ||||||
货期：3-5 working days
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