首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠髓样细胞触发性受体2(TREM2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的TREM2含量。TREM2是一种跨膜受体，主要表达于小胶质细胞，其作用机制涉及感知组织损伤、介导免疫重塑和调控小胶质细胞功能。研究发现，TREM2与多种配体结合后，可激活下游信号通路，影响小胶质细胞增殖、吞噬和炎症反应，从而参与多种疾病如阿尔茨海默病、代谢综合征和肿瘤的病理进程。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，该产品适用于神经免疫机制研究、小胶质细胞功能探索、阿尔茨海默病或代谢相关疾病的动物模型分析等科研场景，为揭示TREM2在病理生理过程中的作用提供可靠工具，实验结果需结合其他研究手段进行综合分析。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：TREM-2, Trem2a, Trem2b, Trem2c, triggering receptor expressed on monocytes 2|triggering receptor expressed on myeloid cells 2a
缩写：TREM2
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat TREM2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 97 | ||||
| Range % | 92-101 | |||||
| 1:2 | Average % | 106 | ||||
| Range % | 101-115 | |||||
| 1:4 | Average % | 88 | ||||
| Range % | 84-92 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 86-98 | |||||
回收率：
| The recovery of rat TREM2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-98 | ||||
| EDTA plasma (n=4) | 92 | 88-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected10002.6602.7542.7072.5305001.9572.0061.9821.8052501.3091.2931.3011.1241250.9190.9370.9280.75162.50.4820.5010.4920.31531.20.4070.4250.4160.23915.60.2470.2380.2430.06600.1740.1800.177 | ||||||
货期：3-5 working days
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