| WB | 1/500-1/1000 | Human,Hamster |
| IF | 咨询技术 | Human,Hamster |
| IHC | 1/50-1/100 | Human,Hamster |
| ICC | 1/50-1/200 | Human,Hamster |
| FCM | 咨询技术 | Human,Hamster |
| Elisa | 咨询技术 | Human,Hamster |
| Aliases | RAD21; HR21; KIAA0078; NXP1; Double-strand-break repair protein rad21 homolog; hHR21; Nuclear matrix protein 1; NXP-1; SCC1 homolog |
| Entrez GeneID | 5885 |
| WB Predicted band size | Calculated MW: 72 kDa; Observed MW: 130 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Hamster |
| Immunogen | A synthetic peptide of human Rad21 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是3篇与Rad21抗体相关的文献摘要信息(基于公开数据模拟，非真实文献)：
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1. **文献名称**: "Structural basis of cohesin cleavage by separase"
**作者**: Shi Z, et al.
**摘要**: 该研究利用Rad21特异性抗体，通过冷冻电镜解析了黏连蛋白亚基Rad21与分离酶(separase)的相互作用机制，揭示了Rad21在姐妹染色单体分离过程中的蛋白水解位点。
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2. **文献名称**: "Cohesin subunit Rad21 regulates neural development through chromatin organization"
**作者**: Smith J, Doe R.
**摘要**: 在小鼠模型中，通过Rad21抗体进行ChIP-seq分析，发现Rad21通过调控染色质三维结构参与神经干细胞分化和大脑发育，其缺失导致神经发育异常。
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3. **文献名称**: "Rad21 antibody-based screening identifies therapeutic vulnerabilities in cancer"
**作者**: Lee H, et al.
**摘要**: 研究团队开发了一种高特异性Rad21单克隆抗体，用于检测多种癌症细胞系中Rad21的表达水平，发现Rad21低表达的肿瘤对DNA损伤类药物(如PARP抑制剂)敏感性显著升高。
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*注：以上文献信息为模拟生成，实际引用需查询PubMed或专业数据库(如PMID编号)。*
The Rad21 antibody is a crucial tool in studying the Cohesin complex, a multi-subunit protein assembly essential for sister chromatid cohesion, DNA repair, and transcriptional regulation. Rad21 (also known as Scc1) is a core component of Cohesin, forming a tripartite ring structure with SMC1 and SMC3 to mediate chromatin interactions. Its role in maintaining genomic stability links it to critical cellular processes, including mitosis, DNA damage response, and gene expression. Dysregulation of Rad21 and Cohesin subunits is associated with developmental disorders (e.g., Cornelia de Lange syndrome) and cancers, highlighting its biomedical relevance.
Rad21-specific antibodies enable researchers to investigate Cohesin dynamics across the cell cycle, localization on chromosomes, and interactions with regulatory factors. These antibodies are widely used in techniques like chromatin immunoprecipitation (ChIP), immunofluorescence, and Western blotting to map Cohesin binding sites, assess protein expression levels, or visualize subcellular distribution. Phospho-specific Rad21 antibodies further distinguish post-translational modifications critical for Cohesin’s dissociation during mitosis. Validation across species (human, mouse, etc.) and compatibility with diverse experimental models (cell lines, tissues) make Rad21 antibodies versatile in both basic and translational research. Their application continues to uncover mechanistic insights into genome organization, replication fidelity, and disease-associated Cohesinopathies.
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