首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠降钙素原(PCT)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的PCT含量。PCT即降钙素原，在细菌感染等引发的全身炎症反应中会显著升高。作为一种新型炎症标志物。研究机制主要是在感染时，细菌内毒素及炎性因子刺激其大量合成，可用于鉴别细菌与病毒感染、评估感染严重程度等。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，适用于体外实验中对不同来源样本的PCT水平检测。研究者可借助探索炎症相关疾病的分子机制、细菌感染模型的动态变化，或评估抗炎药物、免疫调节剂的干预效果。可为感染免疫学、药理学等领域的实验提供可靠数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：PCT
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.8 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat PCT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 84 | ||||
| Range % | 80-88 | |||||
| 1:2 | Average % | 96 | ||||
| Range % | 91-103 | |||||
| 1:4 | Average % | 97 | ||||
| Range % | 91-105 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 89-99 | |||||
回收率：
| The recovery of rat PCT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 101 | 98-107 | ||||
| EDTA plasma (n=4) | 97 | 92-105 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected20002.6552.5542.6052.51610001.8281.7271.7781.6895000.9730.9960.9850.8962500.5480.5590.5540.4651250.3020.3120.3070.21862.50.2440.2330.2390.15031.250.1470.1420.1450.05600.0890.0880.089 | ||||||
货期：3-5 working days
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