首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠血纤蛋白(fibrin)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的fibrin含量。Fibrin即纤维蛋白，是凝血过程的关键产物。其背景与止血和血栓形成密切相关。研究机制上，聚焦于其在凝血级联反应中的生成过程，以及与血小板相互作用形成血栓的机制，还探索其在疾病如心血管病、炎症等病理过程中的作用。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，可用于研究凝血机制异常、心血管疾病模型中的纤维蛋白沉积动态，或评估抗血栓药物对纤维蛋白生成的干预效果，亦可用于炎症性疾病或组织损伤模型中纤维蛋白代谢通路的分子机制探索。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：fibrin
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse fibrin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 93 | ||||
| Range % | 87-103 | |||||
| 1:200 | Average % | 96 | ||||
| Range % | 86-102 | |||||
| 1:400 | Average % | 92 | ||||
| Range % | 84-100 | |||||
| 1:800 | Average % | 94 | ||||
| Range % | 85-100 | |||||
回收率：
| The recovery of mouse fibrin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 97 | 89-101 | ||||
| EDTA plasma (n=4) | 95 | 89-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected10002.7662.6842.7252.6035001.8381.9021.8701.7482501.1231.0851.1040.9821250.6650.6360.6510.52962.50.3840.3650.3750.25331.250.2830.3010.2920.17015.60.1990.2050.2020.08000.1240.1200.122 | ||||||
货期：3-5 working days
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