首页 / 产品 / ELISA试剂盒 / 其他ELISA试剂盒
产品描述：猪血小板衍生生长因子AB(PDGF-AB)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的PDGF-AB含量。PDGF - AB是一种血小板衍生生长因子，在细胞生长、增殖和分化等过程中起重要作用。其异常表达与肿瘤、纤维化等疾病的发生发展相关。研究机制聚焦于其信号通路，通过干预该信号通路有望为相关疾病的治疗提供新策略。试剂盒检测范围为0.312 ng/mL-20 ng/mL，适用于猪模型研究中PDGF - AB的动态监测，例如在创伤修复、心血管疾病机制或干细胞分化等基础科研领域，为探索生长因子网络调控提供有效工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
种属：Sus scrofa (Pig)
样本类型：serum, plasma, tissue homogenates
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of pig PDGF-AB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 95 | |||||
| Range % | 85-103 | ||||||
| 1:2 | Average % | 90 | |||||
| Range % | 85-99 | ||||||
| 1:4 | Average % | 93 | |||||
| Range % | 84-97 | ||||||
| 1:8 | Average % | 95 | |||||
| Range % | 83-100 | ||||||
回收率：
| The recovery of pig PDGF-AB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 95 | 83-101 | |||||
| EDTA plasma (n=4) | 93 | 87-100 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected202.4102.4532.4322.250101.5731.5651.5691.38750.9880.9970.9930.8112.50.6090.6210.6150.4331.250.4290.4380.4340.2520.6250.3470.3350.3410.1590.3120.2450.2420.2440.06200.1800.1830.182 | |||||||
货期：3-5 working days
×
