首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠Ⅱ型胶原(Col Ⅱ)抗体酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的Col Ⅱ Ab含量。试剂盒检测范围为0.312 U/mL-20 U/mL，适用于科研领域对骨关节疾病模型的机制研究；适用于评估关节炎动物模型中Col Ⅱ抗体的表达水平，探究软骨退行性病变的分子机制，或用于筛选调节软骨代谢的候选化合物；为软骨生物学、病理学及药物开发研究提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：collagen type 2(Col 2) antibody,_x000D_ collagen type II(Col II) antibody
缩写：Col Ⅱ Ab
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma
检测范围：0.312 U/mL-20 U/mL
灵敏度：0.078 U/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat Col Ⅱ antibody in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 89 | |||||
| Range % | 86-99 | ||||||
| 1:2 | Average % | 95 | |||||
| Range % | 91-100 | ||||||
| 1:4 | Average % | 93 | |||||
| Range % | 85-100 | ||||||
| 1:8 | Average % | 93 | |||||
| Range % | 82-98 | ||||||
回收率：
| The recovery of rat Col Ⅱ antibody spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 96 | 91-110 | |||||
| EDTA plasma (n=4) | 98 | 89-100 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| U/mlOD1OD2AverageCorrected 202.6072.7252.6662.498 102.0362.0812.0591.891 51.3281.3691.3491.181 2.50.8110.8250.8180.650 1.250.4360.4210.4290.261 0.6250.3490.3600.3550.187 0.3120.2430.2320.2380.070 00.1690.1670.168 | |||||||
货期：3-5 working days
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