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产品描述：牛脂多糖/内毒素(LPS)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的LPS含量。LPS即脂多糖，是革兰氏阴性菌细胞壁的主要成分。其具有免疫原性，可激活免疫系统。研究机制主要围绕其与免疫细胞表面受体结合，触发信号传导通路，导致炎症介质释放等，在感染性疾病、炎症相关研究中有重要意义。试剂盒检测范围为0.312 ng/mL-20 ng/mL，适用于动物模型（如牛、小鼠等）中LPS水平的动态监测，例如评估细菌感染后内毒素释放量、炎症反应程度或药物干预效果等研究；可为体外实验或动物实验提供可靠的数据支持，助力科研人员在免疫学、微生物学及药理学等领域探索LPS相关分子机制。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：LPS
种属：Bos taurus (Bovine)
样本类型：serum, plasma, tissue homogenates
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of bovine LPS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 99 | ||||
| Range % | 93-104 | |||||
| 1:2 | Average % | 102 | ||||
| Range % | 98-110 | |||||
| 1:4 | Average % | 90 | ||||
| Range % | 85-94 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 90-99 | |||||
回收率：
| The recovery of bovine LPS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 85 | 80-90 | ||||
| EDTA plasma (n=4) | 89 | 84-93 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected202.1822.0122.0971.959101.7761.8031.7901.65251.4041.4921.4481.3102.51.0391.0741.0570.9191.250.7410.7240.7330.5950.6250.5410.5190.5300.3920.3120.3030.2790.2910.15300.1390.1370.138 | ||||||
货期：3-5 working days
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