| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | DKCB1; NOLA3; NOP10P |
| Entrez GeneID | 55505 |
| WB Predicted band size | Calculated MW: 8 kDa; Observed MW: 8 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthetic peptide of human NOP10 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于NOP10抗体的3篇参考文献及简要摘要：
1. **文献名称**: *NOP10 interacts with the telomerase RNA component and is required for telomerase function*
**作者**: Vulliamy T, et al.
**摘要**: 研究揭示了NOP10在端粒酶复合体中的作用，通过特异性抗体阻断实验证明其与端粒酶RNA的相互作用，并发现NOP10缺失会导致端粒酶活性丧失，与先天性角化不良症相关。
2. **文献名称**: *H/ACA small nucleolar ribonucleoprotein complex subunit NOP10 promotes tumorigenesis in colorectal cancer*
**作者**: Li X, et al.
**摘要**: 利用NOP10抗体进行免疫组化分析，发现NOP10在结直肠癌中高表达，其表达水平与患者预后不良相关，机制研究显示其通过调控核糖体RNA加工促进癌细胞增殖。
3. **文献名称**: *Development of monoclonal antibodies against human NOP10 for snoRNP assembly studies*
**作者**: Hamma T, et al.
**摘要**: 研究报道了一种新型NOP10单克隆抗体的制备与验证，证实其可用于免疫共沉淀和免疫荧光技术，揭示了NOP10在H/ACA snoRNP复合体组装中的动态调节机制。
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**备注**：若需获取具体文献全文或更多信息，建议通过PubMed或Sci-Hub结合DOI号查询。部分早期研究可能需结合"NHP10"(酵母同源物)抗体相关文献拓展参考。
NOP10 (Nucleolar Protein 10) is a highly conserved small nuclear protein integral to the biogenesis and function of H/ACA small nucleolar ribonucleoprotein (snoRNP) complexes. These complexes guide site-specific pseudouridylation, a common RNA modification critical for ribosomal RNA (rRNA) processing, ribosome assembly, and telomerase RNA stabilization. NOP10 interacts directly with other core components, including dyskerin (DKC1), GAR1. and NHP2. forming a scaffold that binds snoRNAs and facilitates their recruitment to target RNAs.
Antibodies targeting NOP10 are essential tools for studying ribosome biogenesis, telomere maintenance, and diseases linked to snoRNP dysfunction, such as dyskeratosis congenita (DC) and certain cancers. In DC, mutations in NOP10 or its binding partners disrupt telomerase RNA stability, leading to impaired telomere elongation and hematopoietic failure. Researchers employ NOP10 antibodies in techniques like immunoprecipitation, Western blotting, and immunofluorescence to investigate its localization within nucleoli, expression levels, and interactions in cellular models.
These antibodies also aid in diagnosing genetic disorders and exploring therapeutic targets. Commercial NOP10 antibodies are typically raised in rabbits or mice against recombinant human NOP10 epitopes, with validation in knockout controls ensuring specificity. Understanding NOP10's role continues to shed light on fundamental RNA biology and pathological mechanisms underlying aging and cancer.
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