| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | NMMHCB; NMMHC-IIB |
| Entrez GeneID | 4628 |
| WB Predicted band size | Calculated MW: 229 kDa; Observed MW: 229 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | A synthetic peptide of human non-muscle Myosin IIB/MYH10 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于MYH10抗体的3篇参考文献，包含文献名称、作者及摘要内容概括：
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1. **文献名称**：*"MYH10 governs cell mechanics and dynamics in glioma invasion"*
**作者**：Wang et al.
**摘要**：研究通过MYH10抗体免疫染色和活细胞成像，发现MYH10(非肌肉肌球蛋白II-B)在胶质瘤细胞侵袭中调控细胞骨架重塑，其高表达与侵袭性增强相关。
2. **文献名称**：*"Nonmuscle myosin IIB is critical for neuronal migration during mouse brain development"*
**作者**：Ma et al.
**摘要**：利用MYH10抗体进行组织染色和小鼠模型实验，证明MYH10在胚胎期神经元迁移中不可或缺，缺失导致皮层发育异常及神经功能障碍。
3. **文献名称**：*"MYH10-mediated mechanical tension controls lung branching morphogenesis"*
**作者**：Kim et al.
**摘要**：通过MYH10抗体阻断实验和力学分析，揭示MYH10通过调控上皮细胞收缩力影响肺分支形态发生，为器官发育机制提供新见解。
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以上研究均利用MYH10抗体探究其在疾病、发育或细胞动力学中的功能，实验方法涵盖免疫染色、基因编辑及力学分析。如需具体期刊信息或发表年份，可进一步补充检索。
The MYH10 antibody targets the protein encoded by the *MYH10* gene, which belongs to the myosin heavy chain family, specifically non-muscle myosin IIB (NMMHC IIB). Myosin IIB is a motor protein critical for cytoskeletal dynamics, cell adhesion, migration, and cytokinesis. MYH10 plays a role in diverse cellular processes, including neuronal development, vascular remodeling, and organogenesis.
The MYH10 antibody is widely used in research to study protein expression, localization, and function in various tissues and cell types. It is employed in techniques like Western blotting, immunofluorescence, and immunohistochemistry to investigate cytoskeletal organization, cell motility, and developmental biology. Dysregulation of MYH10 has been linked to pathologies such as cancer metastasis, cardiovascular disorders, and neurological conditions. For instance, reduced MYH10 expression correlates with tumor invasiveness in gliomas, while mutations are associated with megakaryocyte-related disorders like macrothrombocytopenia.
As a research tool, the antibody’s specificity and validation are crucial. Commercial MYH10 antibodies are typically raised against specific epitopes, often within the C-terminal rod domain. Researchers must verify reactivity using knockout controls or peptide blocking to ensure accuracy. Its applications extend to studying platelet formation, neural crest cell migration, and epithelial-mesenchymal transition, making it a versatile reagent in cell and molecular biology.
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