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产品描述：人基质细胞衍生因子1α(SDF1A)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的SDF1A/CXCL12含量。SDF1A/CXCL12是重要的趋化因子。其背景上，在胚胎发育、免疫调节等生理过程中发挥关键作用。研究机制方面，它主要通过与CXCR4等受体结合，激活下游信号通路，参与细胞迁移、增殖和存活调控，在肿瘤、炎症等疾病研究中受关注。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，适用于体外实验中SDF1A表达水平的动态监测，例如探索其在肿瘤转移、缺血再灌注损伤或自身免疫性疾病中的调控机制，亦可用于评估药物干预对趋化因子信号通路的影响。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：12-O-tetradecanoylphorbol 13-acetate repressed protein 1 ELISA Kit; AI174028 ELISA Kit; C-X-C motif chemokine 12 ELISA Kit; Chemokine (C-X-C motif) ligand 12 (stromal cell-derived factor 1) ELISA Kit; Chemokine (C-X-C motif) ligand 12 ELISA Kit; Chemokine CXC motif ligand 12 ELISA Kit; cxcl12 ELISA Kit; hIRH ELISA Kit; hSDF-1 ELISA Kit; Intercrine reduced in hepatomas ELISA Kit; IRH ELISA Kit; OTTHUMP00000019491 ELISA Kit; PBSF ELISA Kit; Pre-B cell growth-stimulating factor ELISA Kit; SCYB12 ELISA Kit; SDF 1 ELISA Kit; SDF-1 ELISA Kit; SDF-1-alpha(3-67) ELISA Kit; SDF-1a ELISA Kit; SDF-1b ELISA Kit; SDF1_HUMAN ELISA Kit; SDF1A ELISA Kit; SDF1B ELISA Kit; Stromal cell-derived factor 1 ELISA Kit; Stromal cell-derived factor 1 delta ELISA Kit; Stromal cell-derived factor 1 gamma ELISA Kit; Stromal cell-derived factor 1a ELISA Kit; Stromal cell-derived factor-1 alpha ELISA Kit; Thymic lymphoma cell-stimulating factor ELISA Kit; Tlsf ELISA Kit; TLSF-a ELISA Kit; TLSF-b ELISA Kit; Tlsfa ELISA Kit; Tlsfb ELISA Kit; TPAR1 ELISA Kit
缩写：SDF1A
Uniprot No.：P48061
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.81 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human SDF1A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:5 | Average % | 88 | ||||
| Range % | 85-92 | |||||
| 1:10 | Average % | 96 | ||||
| Range % | 91-102 | |||||
| 1:20 | Average % | 92 | ||||
| Range % | 86-98 | |||||
| 1:40 | Average % | 90 | ||||
| Range % | 85-96 | |||||
回收率：
| The recovery of human SDF1A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 87-96 | ||||
| EDTA plasma (n=4) | 95 | 90-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected20002.7502.7322.7412.64010002.1442.1362.1402.0395001.1631.1441.1541.0532500.6300.6280.6290.5281250.3480.3240.3360.23562.50.2600.2460.2530.15231.250.2100.2010.2060.10500.1050.0970.101 | ||||||
本试剂盒所含材料：
A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human SDF1A antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
One vial Biotin-labeled SDF1A antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
本试剂盒不含材料：
A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
货期：3-5 working days
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