首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠γ分泌酶酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的APH1B含量。APH1B 是一种重要靶点。它在γ-分泌酶复合物中起关键作用。研究发现其与阿尔茨海默病等神经退行性疾病机制相关，可能参与调节淀粉样蛋白生成。目前围绕它探索新治疗策略，深入了解其作用机制或为相关疾病防治带来新思路。试剂盒检测范围为7.8 pg/mL-500 pg/mL，适用于脑组织样本分析、疾病模型构建、神经退行机制探究等科研领域，可支持药物开发中酶活性抑制剂的功效评估、转基因动物模型验证及病理状态下γ分泌酶表达水平动态监测等实验需求，为探索γ分泌酶在细胞信号传导和疾病发生中的功能提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Aph1b; Gamma-secretase subunit APH-1B; APH-1b; Aph-1beta
缩写：APH1B
Uniprot No.：Q8C7N7
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：7.8 pg/mL-500 pg/mL
灵敏度：1.95 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse γ-Secretase in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 96 | ||||
| Range % | 90-102 | |||||
| 1:2 | Average % | 102 | ||||
| Range % | 97-107 | |||||
| 1:4 | Average % | 89 | ||||
| Range % | 87-93 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 90-98 | |||||
回收率：
| The recovery of mouse γ-Secretase spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 103 | 99-106 | ||||
| EDTA plasma (n=4) | 95 | 89-102 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected5002.2342.3562.2952.1302501.3671.3781.3731.2081250.8100.8240.8170.65262.50.4920.4520.4720.30731.20.3130.3060.3100.14515.60.2360.2500.2430.0787.80.2050.2090.2070.04200.1600.1690.165 | ||||||
货期：3-5 working days
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