首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠L-乳酸脱氢酶A链(LDH-A)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的LDHA含量。LDHA是重要的靶点。它在细胞代谢尤其是糖酵解中发挥关键作用，与肿瘤细胞的能量供应和增殖密切相关。研究聚焦于其催化机制及在疾病发生发展中的作用，旨在开发能抑制其活性的药物，为肿瘤等疾病治疗提供新策略。试剂盒检测范围为0.156 mU/mL-10 mU/mL，该产品可广泛应用于肿瘤代谢重编程机制研究、缺血再灌注损伤模型中的乳酸代谢动态监测、运动生理学能量转换研究等领域，特别适用于比较不同处理条件下（如药物干预、基因敲除或过表达）样本中LDH-A活性的差异分析。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Ldha ELISA Kit; Ldh-1 ELISA Kit; Ldh1L-lactate dehydrogenase A chain ELISA Kit; LDH-A ELISA Kit; EC 1.1.1.27 ELISA Kit; LDH muscle subunit ELISA Kit; LDH-M ELISA Kit
缩写：LDHA
Uniprot No.：P06151
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：0.156 mU/mL-10 mU/mL
灵敏度：0.039 mU/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse LDH-A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:100 | Average % | 100 | |||||
| Range % | 95-106 | ||||||
| 1:200 | Average % | 88 | |||||
| Range % | 84-93 | ||||||
| 1:400 | Average % | 107 | |||||
| Range % | 103-110 | ||||||
| 1:800 | Average % | 86 | |||||
| Range % | 83-90 | ||||||
回收率：
| The recovery of mouse LDH-A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 101 | 96-108 | |||||
| EDTA plasma (n=4) | 85 | 81-90 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| mU/mlOD1OD2Average100.1440.1480.14650.2150.2110.2132.50.3250.3310.3281.250.5080.4990.5040.6250.7630.7780.7710.3121.2301.2411.2360.1561.5611.5701.56602.1802.0342.107 | |||||||
货期：3-5 working days
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