WB | 1/500-1/1000 | Human,Mouse,Rat |
IF | 咨询技术 | Human,Mouse,Rat |
IHC | 咨询技术 | Human,Mouse,Rat |
ICC | 技术咨询 | Human,Mouse,Rat |
FCM | 咨询技术 | Human,Mouse,Rat |
Elisa | 咨询技术 | Human,Mouse,Rat |
Aliases | JDP1; HPANBH4 |
Entrez GeneID | 56521 |
WB Predicted band size | Calculated MW: 23 kDa; Observed MW: 23 kDa |
Host/Isotype | Rabbit IgG |
Antibody Type | Primary antibody |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human |
Immunogen | A synthetic peptide of human DNAJC12 |
Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于DNAJC12抗体的模拟参考文献示例(文献内容为虚构,仅供示例参考):
1. **文献名称**: "DNAJC12 as a Novel Biomarker in Parkinson’s Disease: Immunohistochemical Analysis"
**作者**: Smith A, et al.
**摘要**: 本研究通过免疫组化技术,利用DNAJC12抗体检测其在帕金森病患者脑组织中的表达,发现DNAJC12在神经元中的表达显著降低,提示其可能参与神经保护机制。
2. **文献名称**: "Role of DNAJC12 in Protein Quality Control: Antibody-Based Functional Studies"
**作者**: Chen L, et al.
**摘要**: 通过Western blot和免疫荧光技术,验证DNAJC12抗体特异性,并证明DNAJC12与HSP70协同作用,促进错误折叠蛋白的降解,维持细胞稳态。
3. **文献名称**: "DNAJC12 Antibody Validation in Cancer Cell Lines: Implications for Tumor Progression"
**作者**: Gupta R, et al.
**摘要**: 研究利用商业化DNAJC12抗体分析多种癌细胞系,发现其在乳腺癌中高表达,且沉默DNAJC12可抑制肿瘤生长,提示其作为潜在治疗靶点。
4. **文献名称**: "DNAJC12 Deficiency and Metabolic Disorders: Insights from Knockout Mouse Models"
**作者**: Tanaka K, et al.
**摘要**: 使用DNAJC12抗体检测基因敲除小鼠模型的组织样本,发现DNAJC12缺失导致苯丙氨酸代谢异常,为遗传性代谢疾病机制提供了新证据。
(注:以上文献为示例性内容,实际研究中需通过学术数据库检索真实文献。)
DNAJC12 antibody is a research tool designed to detect and study the DNAJC12 protein, a member of the HSP40/DNAJ family of molecular chaperones. DNAJC12. also known as J-domain-containing cochaperone, functions as a co-chaperone for HSP70 proteins, assisting in protein folding, translocation, and degradation. It plays roles in cellular stress responses, neurotransmitter regulation, and metabolic pathways, particularly in phenylalanine metabolism. Mutations in the DNAJC12 gene have been linked to neurological disorders, including early-onset parkinsonism, dystonia, and hyperphenylalaninemia, making it a protein of interest in neurodegeneration and metabolic disease research.
The antibody is widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to investigate DNAJC12 expression patterns across tissues. Studies focus on its involvement in protein quality control systems, interactions with HSP70 in neurodegenerative conditions like Alzheimer's and Parkinson's diseases, and its potential role in cancer progression through EGFR/HER2 signaling pathways. Researchers also utilize DNAJC12 antibodies to explore its diagnostic potential as a biomarker and therapeutic target. Multiple clones (e.g., EPR23047-4) have been developed, validated in knockout cell lines, and optimized for specific applications. Proper validation remains crucial due to potential cross-reactivity with other J-domain proteins and tissue-specific expression variations.
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