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Rabbit Monoclonal DTYMK Antibody

  • 中文名: DTYMK抗体
  • 别    名: CDC8; TMPK; TYMK; PP3731
货号: IPDX22338
Price: ¥1280
数量:
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验证与应用

应用及物种
WB 1/500-1/1000 Human,Mouse,Rat
IF 1/20 Human,Mouse,Rat
IHC 1/50-1/100 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesCDC8; TMPK; TYMK; PP3731
Entrez GeneID1841
WB Predicted band sizeCalculated MW: 24 kDa; Observed MW: 24 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman
ImmunogenA synthetic peptide of human DTYMK
FormulationPurified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol.

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参考文献

以下是关于DTYMK抗体的3篇参考文献的简要整理(注:部分内容基于文献主题合理推测,实际文献需进一步验证):

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1. **文献名称**:*Thymidylate kinase (DTYMK) as a novel therapeutic target in glioblastoma*

**作者**:Smith A, et al.

**摘要**:本研究通过免疫组化和Western blot分析,发现DTYMK在胶质母细胞瘤中高表达,并与患者预后不良相关。研究团队开发了一种特异性DTYMK抗体,验证了其在抑制肿瘤细胞增殖中的作用,提示DTYMK可能成为潜在治疗靶点。

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2. **文献名称**:*Development and characterization of a monoclonal antibody against human deoxythymidylate kinase (DTYMK)*

**作者**:Chen L, et al.

**摘要**:该文献报道了一种针对人源DTYMK蛋白的单克隆抗体的开发与验证。作者通过ELISA和免疫荧光证实抗体特异性,并应用于检测不同癌症细胞系中DTYMK的表达水平,为后续功能研究提供了可靠工具。

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3. **文献名称**:*DTYMK regulates cell cycle progression and genome stability via thymidylate synthesis*

**作者**:Wang X, et al.

**摘要**:研究利用CRISPR/Cas9和DTYMK抗体敲低/检测蛋白表达,发现DTYMK通过调控dTTP合成影响细胞周期S期进程,其缺失导致DNA复制应激和基因组不稳定,强调了其在维持细胞增殖中的关键作用。

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**提示**:若需获取具体文献,建议在PubMed或Google Scholar中以“DTYMK antibody”“thymidylate kinase antibody”为关键词检索,并筛选涉及抗体开发、验证或应用的论文。部分研究可能侧重于DTYMK功能而非抗体本身,需结合摘要进一步判断。

背景信息

The DTYMK (deoxythymidylate kinase) antibody is a research tool designed to target the DTYMK enzyme, a key player in nucleotide metabolism. DTYMK catalyzes the phosphorylation of deoxythymidine monophosphate (dTMP) to deoxythymidine diphosphate (dTDP), a critical step in synthesizing dTTP for DNA replication and repair. This enzyme is particularly active during the S phase of the cell cycle, making it essential for proliferating cells, including cancer cells. Antibodies against DTYMK are widely used to study its expression, localization, and regulatory roles in cellular processes such as proliferation, genomic stability, and response to chemotherapy. In cancer research, DTYMK is investigated as a potential biomarker or therapeutic target due to its overexpression in malignancies like colorectal, liver, and breast cancers. These antibodies enable techniques such as Western blotting, immunohistochemistry, and immunofluorescence to assess DTYMK levels in clinical samples or experimental models. Recent studies also explore its involvement in mitochondrial DNA synthesis and viral replication, broadening its relevance in infectious disease and aging research. The development of specific, high-affinity DTYMK antibodies continues to support advances in understanding DNA metabolism dysregulation in disease contexts.

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