| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | DAGK5; DAGK6; DGK-ZETA; hDGKzeta |
| Entrez GeneID | 8525 |
| WB Predicted band size | Calculated MW: 104 kDa; Observed MW: 124,114 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Recombinant protein of human DGKZ/DGK-zeta |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于DGKZ抗体的3篇参考文献示例(注：以下为模拟内容，实际文献需通过学术数据库查询确认)：
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1. **文献名称**：*DGKζ regulates T-cell receptor signaling by modulating diacylglycerol turnover*
**作者**：Yasuda S, et al.
**摘要**：本研究利用特异性DGKZ抗体，发现DGKζ通过将DAG转化为PA，负调控T细胞受体(TCR)信号传导。实验表明，DGKζ缺失会增强DAG介导的Ras/ERK通路激活，影响T细胞增殖。
2. **文献名称**：*Expression profiling of DGK isoforms in glioblastoma using a novel monoclonal antibody panel*
**作者**：Yuan J, et al.
**摘要**：通过开发高特异性DGKZ单克隆抗体，分析了胶质母细胞瘤中DGK亚型的表达模式。结果显示DGKZ高表达与肿瘤侵袭性相关，提示其作为潜在预后标志物的价值。
3. **文献名称**：*Role of DGKζ in synaptic plasticity: Evidence from antibody-based localization studies*
**作者**：Hozumi Y, et al.
**摘要**：采用免疫组化和Western blot技术，结合DGKZ抗体，证实DGKζ在小鼠海马神经元突触后膜富集，调控PA依赖性信号通路，影响突触可塑性和学习记忆功能。
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如需真实文献，建议在PubMed或Web of Science中检索关键词“DGKZ antibody”或“diacylglycerol kinase zeta”。部分研究可能涉及抗体在疾病机制、信号通路或诊断中的应用。
DGKZ (Diacylglycerol kinase zeta) is a member of the diacylglycerol kinase (DGK) family, which comprises enzymes that phosphorylate diacylglycerol (DAG) to produce phosphatidic acid (PA). Both DAG and PA are critical lipid secondary messengers involved in signaling pathways regulating cell proliferation, apoptosis, and membrane trafficking. DGKZ, specifically, is a type IV DGK isoform characterized by its unique structural domains, including a pleckstrin homology (PH) domain, a sterile alpha motif (SAM), and nuclear localization signals, distinguishing it from other isoforms. It localizes to the nucleus and cytoplasm, suggesting dual roles in modulating nuclear signaling and cytoplasmic lipid metabolism.
DGKZ antibodies are essential tools for studying the expression, localization, and functional mechanisms of DGKZ in cellular processes. Researchers employ these antibodies in techniques like Western blotting, immunofluorescence, and immunoprecipitation to explore DGKZ's involvement in immune responses, neuronal development, and cancer. Dysregulation of DGKZ has been linked to diseases such as bipolar disorder, Alzheimer's disease, and malignancies, where altered DAG/PA signaling disrupts pathways like PKC, mTOR, and Ras. By enabling precise detection of DGKZ, these antibodies aid in elucidating its role in health and disease, offering potential insights for therapeutic targeting. Their specificity and reliability are critical for validating DGKZ interactions and post-translational modifications in diverse experimental models.
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