首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠M型肌酸激酶(CKM)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的CKM含量。试剂盒检测范围为1.56 mU/mL-100 mU/mL，适用于小鼠模型研究中肌肉损伤评估、运动生理学实验或药物干预后能量代谢变化的分析，例如肌肉疾病模型机制研究、运动性肌损伤评价或心肌代谢相关药物的科研筛选，为肌肉相关疾病的病理机制探索提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Ckm ELISA Kit; Ckmm ELISA Kit; Creatine kinase M-type ELISA Kit; EC 2.7.3.2 ELISA Kit; Creatine kinase M chain ELISA Kit; Creatine phosphokinase M-type ELISA Kit; CPK-M ELISA Kit; M-CK ELISA Kit
缩写：CKM
Uniprot No.：P07310
种属：Mus musculus (Mouse)
样本类型：serum, plasma
检测范围：1.56 mU/mL-100 mU/mL
灵敏度：0.39 mU/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse CKM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 90 | ||||
| Range % | 86-95 | |||||
| 1:2 | Average % | 98 | ||||
| Range % | 92-100 | |||||
| 1:4 | Average % | 90 | ||||
| Range % | 87-96 | |||||
| 1:8 | Average % | 91 | ||||
| Range % | 85-95 | |||||
回收率：
| The recovery of mouse CKM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 89-99 | ||||
| EDTA plasma (n=4) | 99 | 95-106 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| mU/mlOD1OD2AverageCorrected1002.8933.1123.0032.890502.3232.3942.3592.246251.4871.5551.5211.40812.50.9020.9800.9410.8286.250.4310.4080.4200.3073.120.3470.3210.3340.2211.560.2480.2600.2540.14100.1150.1100.113 | ||||||
货期：3-5 working days
×
